ExactAb™,PBS Only,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Application
WB: 1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa
ICC/IF: 1/200
Flow: 1/200 - 1/25,000
Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 16.0 s
Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 71 kDa
Exposure time: 2.3 s
Grp75 Mouse mAb (Ab181865) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Grp75 Mouse mAb (Ab181865) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).
Grp75 Mouse mAb (Ab181865) - ICC/IF
IF analysis of Grp75 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Grp75 Mouse mAb (Ab181865) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
1/200
1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa
1/200 - 1/25,000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0404640 |
分析证书 | Ab181865 |
| ZJ24F0404641 |
分析证书 | Ab181865 |
| ZJ24F0404642 |
分析证书 | Ab181865 |
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