Recombinant full length protein corresponding to Human COX IV
阳性对照
WB: HeLa, HepG2, A431, Jurkat, MCF-7, THP-1, K562, HEK293 and A549 Whole cell Lysates ICC/IF: HeLa cells
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
AB03/3G1
Format
Whole IgG
亚型
IgG1
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
biological_products
储存与运输
物理外观
Liquid
储存缓冲液
PBS, pH 7.2
防腐剂
NO
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
图片
COX IV Mouse mAb (Ab181867) - Western Blot All lanes: COX IV Mouse Antibody (Ab181867) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 19.6 kDa Observed band size: 17.4 kDa Exposure time: 112.6 s
COX IV Mouse mAb (Ab181867) - Western Blot All lanes: COX IV Mouse Antibody (Ab181867) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 19.6 kDa Observed band size: 17.2 kDa Exposure time: 13.7 s
COX IV Mouse mAb (Ab181867) - ICC/IF IF analysis of COX IV (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with COX IV Mouse mAb (Ab181867) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
ZJ24F0404646
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