ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human COX IV Antibody, Recombinant antibody (RR684), could be used for WB, IHC, Flow, IF, ICC, IP and so on.
Application:
WB: 1/5000 - 1/10000
IHC: 1/200 - 1/400
Flow: 1/200 - 1/1000
IF/ICC: 1/10 - 1/500
IP: 1/50
Recombinant COX IV Antibody (Ab097410) - Western Blot
All lanes: Recombinant COX IV Antibody (Ab097410) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 18 kDa
Exposure time: 15.1 s
Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant COX IV Antibody (Ab097410) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of liver tissue labelling COX IV with Recombinant COX IV Antibody (Ab097410) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant COX IV Antibody (Ab097410) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant COX IV Antibody (Ab097410)(Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant COX IV Antibody (Ab097410) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant COX IV Antibody (Ab097410) - IP
COX IV was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant COX IV Antibody (Ab097410) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant COX IV Antibody (Ab097410) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant COX IV Antibody (Ab097410) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).
Exposure: 20.0 s
Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
1/50
1/5000 – 1/10000
1/200 - 1/400
1/200 - 1/1000
1/10 - 1/500
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0911325 |
分析证书 | Ab097410 |
| ZJ23F0901104 |
分析证书 | Ab097410 |
| ZJ23F0901105 |
分析证书 | Ab097410 |
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