Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity).
Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
宿主种属
小鼠(Mouse)
特异性
Human Cytokeratin 5
种属反应性
人(Human)
免疫原
Recombinant protein corresponding to Human Cytokeratin 5
阳性对照
WB: A431, HeLa, HepG2 and SK-OV-3 whole cell lysates IF/ICC: HeLa cells
偶联
Unconjugated
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Cytokeratin 5 Mouse mAb (Ab183857) - Western Blot All lanes: Cytokeratin 5 Mouse Antibody (Ab183857) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 58,65 kDa Exposure time: 44.1 s
Cytokeratin 5 Mouse mAb (Ab183857) - ICC/IF IF analysis of Cytokeratin 5 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cytokeratin 5 Mouse mAb (Ab183857) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
ZJ25F0117077
危险品化学品经营许可证（带存储）