Recombinant CD16/CD32 Antibody, clone P24A10, could be used for Flow Cytometry, ELISA, Blocking and so on.
规格
产品名称
Recombinant CD16/CD32 Antibody
别名
FcγIII受体|FcγII受体|白细胞分化抗原16|白细胞分化抗原32|CD16/32抗体
英文别名
CD32|Fcgr2|Fcr-2|Fcr-3|Ly-17|LyM-1|fcRII|FcgRII|Fcgr2a|Ly-m20|F630109E10Rik|CD16|CD16A|FCG3|FCGR3|FCGR3A|FCGRIII|FcGRIIIA|FCR-10|FCRIII|FCRIIIA|IGFR3|IMD20|Low affinity immunoglobulin gamma Fc region receptor III-A|Fc gamma RII/RIII|CD16a antibody|CD16b antibody|CD32 antibody|CD32A antibody|CD32B antibody|CDw32 antibody|Fc fragment of IgG low affinity IIa receptor antibody|Fc fragment of IgG low affinity IIb receptor antibody|Fc fragment of IgG low affinity IIIa receptor antibody|Fc fragment of IgG low affinity IIIb receptor antibody|Fc gamma receptor III A antibody|Fc gamma receptor IIIb antibody|Fc gamma RIIa antibody|Fc gamma RIIb antibody|Fc gamma RIII beta antibody|Fc-gamma RII-a antibody|Fc-gamma-RIIa antibody|FcGR antibody|FCGR2A antibody|FCGR2B antibody|FCGR3 antibody|FCGR3A antibody|FCGR3B antibody|FcRII-a antibody|FcRIII antibody|IgG Fc receptor II-a antibody|Low affinity immunoglobulin gamma Fc region receptor II-a antibody
规格或纯度
无动物源,无载体,重组,ExactAb™,无叠氮钠,已验证,高性能,PBS Only,≥95%(SDS-PAGE),See COA
生化机理
CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b.
Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.
CD32: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
宿主种属
大鼠(Rat)
特异性
Mouse CD16/CD32
种属反应性
小鼠(Mouse)
免疫原
Sorted pre-B cells
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein A purified
浓度
See COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant CD16/32 Antibody (Ab211677) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD16/32 with Recombinant CD16/32 Antibody (Ab211677) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
Recombinant CD16/32 Antibody (Ab211677) - Flow Cytometry Flow cytometry analysis of the expression of CD16/32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining. Left: Flow cytometry analysis of CD16/32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD16/32 Antibody (Ab211677) at 0.2 μg/mL for 1 hour at 4°C. Blue - Rat IgG (Biotin) (Ab180310) . Black - Unlabelled control, cells without incubation with primary antibody. Right: C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) blocked with purified Recombinant CD16/32 Antibody (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with rat IgG (PE) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.
Recombinant CD16/32 Antibody (Ab211677) - SEC The purity of Recombinant CD16/32 Antibody (Ab211677) is more than 95% verified by HPLC.
应用
应用名称
稀释比例
Flow Cytometry
≤0.1 µg per million cells in 100 µL volume
Blocking
1.0 µg per million cells in 100 µL volume
高级数据
关联靶点（人）
FCGR2ATbio低亲和力免疫球蛋白γFc区受体II-a(Low affinity immunoglobulin gamma Fc region receptor II-a)
(0 活性数据)
ZJ25F0319694
危险品化学品经营许可证（带存储）