CD16 and CD32 serve as low affinity receptors for IgG Fc constant regions and are involved in regulating various cellular functions including antibody-dependent cellular toxicity (ADCC), phagocytosis, effector cell degranulation, and B cell proliferation.
宿主种属
大鼠(Rat)
特异性
CD16/32
种属反应性
小鼠(Mouse)
阳性对照
Flow Cytometry: C57BL/6 splenocytes
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
防腐剂
No
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant CD16/CD32 Antibody (Ab210978) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD16/CD32 with Recombinant CD16/CD32 Antibody (Ab210978) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
Recombinant CD16/CD32 Antibody (Ab210978) - Flow Cytometry Flow cytometry analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining. Left: Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with recombinant CD16/CD32 antibody (Ab210978) at 0.2 μg/mL for 1 hour at 4°C. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody. Right: C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) blocked with purified recombinant CD16/CD32 antibody (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with rat IgG (PE) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.
Recombinant CD16/CD32 Antibody (Ab210978) - SEC The purity of Recombinant CD16/CD32 Antibody (Ab210978) is more than 95% verified by HPLC.
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