Non-specific binding to Leukocytes is a well-known challenge in flow cytometry. Though the specific mechanism of non-specific binding is not known, the binding of cyanine acceptor fluorophores to CD64 has been implicated in the literature. Mouse FcR Blocking Reagent blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, Receptors in vitro and in vivo. Furthemore, it effectively inhibits non-specific staining by various PE, PerCP, and APC tandem dyes on leukocytes. Mouse FcR Blocking Reagent blocks both clone P90A23 and 2.4G2 raised against mouse CD16/32.
CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b.
Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.
CD32: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
宿主种属
大鼠(Rat)
特异性
Mouse CD16/CD32
种属反应性
小鼠(Mouse)
免疫原
Mouse BALB/c Macrophage J774
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein A purified
浓度
0.2 mg/mL
分子类型
生物试剂/缓冲液
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
Store at 4°C.
图片
Mouse FcR Blocking Reagent (Ab219990) - Flow Cytometry Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with Mouse FcR Blocking Reagent. C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) after being blocked with Mouse FcR Blocking Reagent (Ab219990) (right panel) compared to an no blocking control (left panel). Left panel - No blocking control, cells were stained with Rat IgG (PE) (Ab180312) without any blocking reagent.
Mouse FcR Blocking Reagent (Ab219990) - Flow Cytometry Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with Mouse FcR Blocking Reagent. C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) after being blocked with Mouse FcR Blocking Reagent (Ab219990) (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with Rat IgG (PE) (Ab180312) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.
应用
应用名称
稀释比例
Flow Cytometry
5.0 μL/test. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with Mouse FcR blocking reagent as 5.0 μL per 106 cells in 100 µl volume for 30 minutes at 4℃ prior to immunostai
ZJ26F0636520
危险品化学品经营许可证（带存储）