无动物源,无载体,重组,ExactAb™,无叠氮钠,已验证,高性能,PBS Only,≥95%(SDS-PAGE),See COA
生化机理
CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b.
Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.
CD32: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
宿主种属
大鼠(Rat)
特异性
Mouse CD16/CD32
种属反应性
小鼠(Mouse)
免疫原
Mouse BALB/c Macrophage J774
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
Unconjugated
备注
此产品可替代 A412018
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein A purified
浓度
See COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant CD16/CD32 Antibody (Ab212150) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD16/CD32 with Recombinant CD16/CD32 Antibody (Ab212150) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
Recombinant CD16/CD32 Antibody (Ab212150) - Flow Cytometry Flow cytometry analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining. Left: Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD16/CD32 Antibody (Ab212150) at 0.2 μg/mL for 1 hour at 4°C. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody. Right: C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) blocked with purified Recombinant CD16/CD32 Antibody (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with rat IgG (PE) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.
Recombinant CD16/CD32 Antibody (Ab212150) - SEC The purity of Recombinant CD16/CD32 Antibody (Ab212150) is more than 95% verified by HPLC.
ZJ26F0636517
危险品化学品经营许可证（带存储）