Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.
宿主种属
兔(Rabbit)
特异性
MSH6
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human MSH6 (AA 1-13).
阳性对照
WB: C6 cell lysate. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
PBS, pH 7.4
防腐剂
から
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。
图片
Recombinant MSH6 Antibody (Ab343884) - Western Blot
All lanes: Recombinant MSH6 Antibody (Ab343884) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 169 kDa
Exposure time: 3.3 s
Recombinant MSH6 Antibody (Ab343884) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant MSH6 Antibody (Ab343884) (red), and DAPI (blue).
Recombinant MSH6 Antibody (Ab343884) - IHC
Immunohistochemical analysis of paraffin-embedded human tonsil using Recombinant MSH6 Antibody (Ab343884).
ZJ26F0939268
危险品化学品经营许可证（带存储）