计算溶液所需的质量、体积或浓度。
Recombinant MCM5 Antibody (Ab338641) - Western Blot
All lanes: Recombinant MCM5 Antibody (Ab338641) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 76, 80, 91, 104 kDa
Exposure time: 51.7 s
The apparent molecular weight difference of MCM5 across species is mainly due to species‑specific phosphorylation levels—phosphorylation slows electrophoretic migration, increasing the observed size. Additionally, variations in cell cycle phase or experimental conditions can modulate modification status, causing minor shifts in band positions.
危险品化学品经营许可证(带存储)