计算溶液所需的质量、体积或浓度。
BPTE电泳缓冲液 (10×, RNase Free) 主要由 PIPES、Tris 和 EDTA 等成分组成,是一种常用的 RNA 电泳缓冲液。本产品经 0.1% DEPC 处理,有效去除核酸酶,确保 RNA 在电泳过程中不被降解。使用时需用 DEPC 处理水稀释 10 倍至 1× 工作液。适用于 RNA 变性或非变性琼脂糖凝胶电泳。
本产品仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
取适量 BPTE 电泳缓冲液 (10×),用 DEPC 处理水稀释 10 倍,混匀即得 1× 工作液。
将 1× 工作液倒入电泳槽,进行 RNA 凝胶电泳。
注意事项
实验全程需严格避免 RNase 污染,建议使用 RNase-free 耗材及 DEPC 处理水。
为了您的安全和健康,请穿戴实验服和一次性手套操作。
试剂开封后请尽快使用,并密封保存,以防影响后续实验效果。
BPTE Running Buffer (10×, RNase-Free) is primarily composed of PIPES, Tris, and EDTA. It is a commonly used buffer for RNA electrophoresis. This product is treated with 0.1% DEPC to effectively inactivate nucleases, ensuring RNA integrity during electrophoresis. For use, dilute 10-fold with DEPC-treated water to obtain a 1× working solution. It is suitable for both denaturing and non-denaturing agarose gel electrophoresis of RNA.
This product is for research use only. Not for use in diagnostic procedures or other purposes.
Procedure (For Reference Only)
Take an appropriate amount of BPTE Running Buffer (10×) and dilute it 10-fold with DEPC-treated water. Mix well to obtain the 1× working solution.
Pour the 1× working solution into the electrophoresis tank and proceed with RNA gel electrophoresis.
Notes
Strictly avoid RNase contamination throughout the entire experiment. It is recommended to use RNase-free consumables and DEPC-treated water.
For your safety and health, please wear laboratory appropriate attire and disposable gloves during operation.
Use the reagent as soon as possible after opening and keep it sealed to avoid affecting subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | B1518790 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for molecular biology 级别指南 → 查看 RNase free 级别指南 → 查看 for NA electrophoresis 级别指南 →