计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
墨汁荚膜染色对于观察新型隐球菌有很好的作用,印度墨汁(IndiaInk)属于非常优质的墨汁,新型隐球菌菌体周围存在粘多糖荚膜物,用印度墨汁染色法能证明荚膜的存在。新型隐球菌染色液采用墨汁配方,染色后新型隐球菌的荚膜取代了墨汁中的胶状碳粒,菌体被清楚无色透明的晕圈环绕着。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
离心机、载玻片、盖玻片、显微镜
操作步骤(仅供参考):
1、样本离心,取沉淀涂于载玻片上。
2、在样本涂片处滴加新型隐球菌染色液。
3、加盖盖玻片,并防止气泡产生。
4、用手指轻轻压一下,使样本与新型隐球菌染色液混合变薄。
5、在低倍镜下寻找有荚膜的细菌,并转高倍镜或油镜下仔细辨认。
染色结果:
| 新型隐球菌 | 宽厚透亮的荚膜,细胞壁明显 |
| 背景 | 黑色 |
注意事项:
1、有效期内的已开封新型隐球菌染色液应在6个月内用完,每次用后尽量密封好,以免挥发或变质。
2、新型隐球菌染色液存储时间较长后易产生沉淀,如果镜下发现颗粒粗糙者应过滤后使用或弃用。
3、目的样本尽量新鲜。
4、样本的纯度和浓度应适宜,浓度过低时在镜下很难寻找到新型隐球菌。
5、样本和新型隐球菌染色液分布应均匀。
6、为了您的安全和健康,请穿实验服并戴一次性手套操作。
India ink capsule staining is highly effective for the observation of Cryptococcus neoformans. India ink is a premium-grade ink. A mucopolysaccharide capsule exists around the cell body of Cryptococcus neoformans, and the presence of this capsule can be demonstrated by the India ink staining method. The Cryptococcus neoformans staining solution is formulated based on the India ink recipe. After staining, the capsule of Cryptococcus neoformans displaces the colloidal carbon particles in the ink, leaving the fungal cell surrounded by a distinct, colorless, and transparent halo. This reagent is intended for research use only and is not suitable for clinical diagnosis or any other purposes.
Materials to Be Prepared by User:
Centrifuge, glass slides, cover slips, microscope
Operating Procedures (For Reference Only):
1. Centrifuge the sample and spread the sediment onto a glass slide.
2. Add a drop of Cryptococcus neoformans Staining Solution onto the sample smear.
3. Place a cover slip over the smear and avoid the formation of air bubbles.
4. Press gently with your finger to mix the sample with the staining solution and flatten the smear to a thin layer.
5. Locate the capsulated microorganisms under the low-power objective lens, then switch to the high-power objective lens or oil immersion lens for detailed identification.
Staining Results:
| Cryptococcus neoformans | Thick, wide, transparent capsule with a distinct cell wall |
| Background | Black |
Precautions:
1. Opened Cryptococcus neoformans Staining Solution within its expiration date should be used up within 6 months. Seal the container tightly as much as possible after each use to prevent volatilization or deterioration.
2. Cryptococcus neoformans Staining Solution is prone to precipitation after long-term storage. If coarse particles are observed under the microscope, the solution should be filtered before use or discarded.
3. The target samples should be as fresh as possible.
4. The purity and concentration of the samples should be appropriate; it will be difficult to detect Cryptococcus neoformans under the microscope if the concentration is too low.
5. Ensure uniform distribution of the samples and the staining solution.
6. For your safety and health, wear a lab coat and disposable gloves during the operation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1508784 |
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