The A-431 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
A-431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
规格
产品名称
A-431 Whole Cell Lysate
别名
A-431 全细胞裂解物
英文别名
A431 Whole Cell Lysate
规格或纯度
Carrier Free, Azide Free, Mycoplasma free
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
应用
WB
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
浓度
Mycoplasma free
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
图片
A-431 Whole Cell Lysate (cl155901) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution Lane 1: C6 Whole Cell Lysate (cl155903) Lane 2: A-431 Whole Cell Lysate (cl155901) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 42.0 kDa Observed band size: 42.0 kDa
A-431 Whole Cell Lysate (cl155901) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/1000 dilution Lane 1: C6 Whole Cell Lysate (cl155903) Lane 2: A-431 Whole Cell Lysate (cl155901) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 29.0 kDa Observed band size: 29.0 kDa
A-431 Whole Cell Lysate (cl155901) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution Lane 1: C6 Whole Cell Lysate (cl155903) Lane 2: A-431 Whole Cell Lysate (cl155901) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution Predicted band size: 60.0 kDa Observed band size: 58.0 kDa