The A549 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
A549 cells are used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism. Cells are cytokeratin positive by immunoperoxidase staining. This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male (PubMed 4357758). Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway (PubMed 175022).
规格
产品名称
A549 Whole Cell Lysate
别名
A549 全细胞裂解物
规格或纯度
Carrier Free, Azide Free, 200μg, Mycoplasma free
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
应用
WB
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
浓度
200μg, Mycoplasma free
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
图片
A549 Whole Cell Lysate (cl155906) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
A549 Whole Cell Lysate (cl155906) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
A549 Whole Cell Lysate (cl155906) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 58 kDa
ZJ23F0500182
危险品化学品经营许可证（带存储）