![DiR' [DiIC18(7)],用于膜染色](https://www.aladdin-e.com/media/catalog/product/cache/207e23213cf636ccdef205098cf3c8a3/D/1/D131031.png)
计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
提供 ≥95% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,充氩。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 34 篇同行评审文献引用。
概述
最大吸收波长/发射波长: 748/780 nm
推荐滤光片设置: 710 ex/760 em
图1.从脾脏分离的T细胞用DiR和i.v进行荧光染色。 （5x106个细胞/小鼠）注射到Nu / Nu小鼠中。 上面使用IVIS Spectrum注射后24小时拍摄的图像显示，细胞归巢于脾脏。
DiR染料是亲脂性的近红外花菁荧光染料，可以用来染细胞膜和其它脂溶性生物结构。18个碳的长链插入细胞膜中从而对细胞进行染色, 而细胞间的染料转移可以忽略不计。DiR的发射的是近红外荧光可以穿透细胞和组织，在活体成像中用来示踪。
DIR一般对原代细胞进行荧光染色并可进行活体成像分布观察, (例如下列细胞embryonic stem cells, bone marrow derived stem cells, adipose derived stem cells, lymphocytes and erythrocytes),
图2.通过将25 mg溶于3 mL乙醇来制备DiR储备液。 通过在5 mL PBS中稀释199 µL储备液来制备320 µg / mL的工作溶液。 从脾脏分离的T细胞与320 µg / mL DiR孵育。 孵育30分钟后，将细胞在4ºC下以1000 rpm离心离心3分钟，得到蓝色沉淀。 将细胞在PBS中洗涤两次并静脉内注射（5×106细胞/小鼠）。 对照组在PBS中每只小鼠注射5×106个细胞。 在注射后10分钟，1小时，6小时和24小时用IVIS Spectrum对小鼠成像。 用于DiR成像的理想滤光片组是710 nm激发和760 nm发射。 在所有时间点对小鼠的背侧和腹侧成像。 注射后24小时，收集脑，骨头，脾，肝，肺和肾用于离体成像。
非侵入性体内成像实时显示了向肝脏和脾脏注射的T细胞的归巢过程，这通过离体成像得到了证实。
参考文献
Kalchenko et al., Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. Journal of Biomedical optics, September/October 2006, Vol 11(5).
Product Introduction
Maximum absorption wavelength/emission wavelength: 748/780 nm
Recommended filter setting: 710 ex/760 em
Figure 1. T-cells isolated from the spleen were fluorescently stained with DiR and i.v. injected (5x106 cells/mouse) into a Nu/Nu mouse. Images above taken 24hrs post injection with IVIS Spectrum show cells homing to the spleen.
DiR dye is a lipophilic near-infrared cyanine fluorescent dye that can be used to dye cell membranes and other fat-soluble biological structures. A long chain of 18 carbons is inserted into the cell membrane to stain cells, while the transfer of dye between cells is negligible. DiR emits near-infrared fluorescence that can penetrate cells and tissues and is used for tracking in in vivo imaging.
DIR generally performs fluorescent staining of primary cells and can observe the distribution of in vivo imaging, (For example, the following cell embryonic stem cells, bone marrow derived stem cells, adipose derived stem cells, lymphocytes and erythrocytes),
Figure 2. DiR stock was prepared by dissolving 25 mg in 3 mL ethanol. Working solution of 320 µg/mL was prepared by diluting 199 µL of stock solution in 5 mL PBS. T-cells isolated from the spleen were incubated with 320 µg/mL DiR. After 30 min incubation, cells were spun down for 3 min at 1000 rpm at 4 ºC resulting in a blue pellet. Cells were washed twice in PBS and injected intravenously (5 x 106 cells/mouse). Control group was injected with 5 x 106 cells/mouse in PBS. Mice were imaged with IVIS Spectrum at 10 min, 1hr, 6hr and 24 hrs post injection. Ideal filter set for DiR imaging is 710 nm excitation and 760 nm emission. Mice were imaged dorsally as well as ventrally at all time points. Brain, bones, spleen, liver, lungs and kidneys were harvested for ex vivo imaging 24 hrs post injection.
Non-invasive in vivo imaging showed the homing process of injected T cells to the liver and spleen in real time, which was confirmed by ex vivo imaging.
References
Kalchenko et al., Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. Journal of Biomedical optics, September/October 2006, Vol 11(5).
规格
别名
2-(7-(3,3-二甲基-1-十八烷基吲哚啉-2-亚基)庚-1,3,5-三烯-1-基)-3,3-二甲基-1-十八烷基-3H-吲哚-1-鎓碘化物
英文别名
2-(7-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-dimethyl-1-octadecyl-3H-indol-1-ium iodide
规格或纯度
≥95%
英文名称
DiR' [DiIC18(7)]
储存条件
避光,-20°C储存,充氩
运输条件
超低温运输
纯度
≥95%
名称和识别符
PubChem SID
EC号
144-876-3
分子类型
未知
IUPAC Name
2-[7-(3,3-dimethyl-1-octadecylindol-1-ium-2-yl)hepta-2,4,6-trienylidene]-3,3-dimethyl-1-octadecylindole;iodide
INCHI
1S/C63H101N2.HI/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-38-46-54-64-58-50-44-42-48-56(58)62(3,4)60(64)52-40-36-35-37-41-53-61-63(5,6)57-49-43-45-51-59(57)65(61)55-47-39-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2;/h35-37,40-45,48-53H,7-34,38-39,46-47,54-55H2,1-6H3;1H/q+1;/p-1
InChi Key
JLIOTPLALDYAEH-UHFFFAOYSA-M
Smiles
CCCCCCCCCCCCCCCCCCN1C2=CC=CC=C2C(C1=CC=CC=CC=CC3=[N+](C4=CC=CC=C4C3(C)C)CCCCCCCCCCCCCCCCCC)(C)C.[I-]
Isomeric SMILES
CCCCCCCCCCCCCCCCCCN1C2=CC=CC=C2C(C1=CC=CC=CC=CC3=[N+](C4=CC=CC=C4C3(C)C)CCCCCCCCCCCCCCCCCC)(C)C.[I-]
PubChem CID
分子量
1013.41
技术文档
高级数据
三维结构
交互式化学结构模型
化学和物理性质
溶解性
Solubility：Methanol (Slightly, Heated), Water (Very Slightly, Heated, Sonicated)
敏感性
对光线敏感
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 J2612353 |
分析证书 | D131031 |
| J2612337 |
分析证书 | D131031 |
| J2612336 |
分析证书 | D131031 |
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分析证书 | D131031 |
| A2619453 |
分析证书 | D131031 |
| A2619452 |
分析证书 | D131031 |
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分析证书 | D131031 |
| A2619449 |
分析证书 | D131031 |
| C2401218 |
分析证书 | D131031 |
| C2401221 |
分析证书 | D131031 |
| K2307324 |
分析证书 | D131031 |
| G2511214 |
分析证书 | D131031 |
| G2511213 |
分析证书 | D131031 |
| G2511212 |
分析证书 | D131031 |
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分析证书 | D131031 |
| E2305222 |
分析证书 | D131031 |
| E2305231 |
分析证书 | D131031 |
| E2305210 |
分析证书 | D131031 |
| E2305218 |
分析证书 | D131031 |
| A2515249 |
分析证书 | D131031 |
| A2515250 |
分析证书 | D131031 |
| A2515251 |
分析证书 | D131031 |
| A2515252 |
分析证书 | D131031 |
| J2423547 |
分析证书 | D131031 |
| J2423544 |
分析证书 | D131031 |
| J2423540 |
分析证书 | D131031 |
| G2405314 |
分析证书 | D131031 |
| G2405318 |
分析证书 | D131031 |
| G2405333 |
分析证书 | D131031 |
| C2401220 |
分析证书 | D131031 |
| C2401217 |
分析证书 | D131031 |
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分析证书 | D131031 |
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分析证书 | D131031 |
| K2307313 |
分析证书 | D131031 |
| K2307331 |
分析证书 | D131031 |
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分析证书 | D131031 |
| E2305209 |
分析证书 | D131031 |
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分析证书 | D131031 |
| G2222523 |
分析证书 | D131031 |
| G2222517 |
分析证书 | D131031 |
| G2222503 |
分析证书 | D131031 |
技术文档和文章
Liposome Panorama Guide: Definition, Structural Mechanisms, Applications, and Selection Workflow (Including Products Table 1–Table 3)
目录： 技术文章
Understanding Cyanine Dyes: Structure & Mechanism, Channel Selection, Troubleshooting Essentials, and a Product Selection Roadmap (with 6 Category Tables)
目录： 技术文章
细胞结构荧光染色与多通道成像
目录： 技术文章
药物如何递送？脂质纳米颗粒
目录： 技术文章
此产品的引用文献
溶液计算器
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