BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
一些组织内含有骨质或钙化灶时，含钙的组织不宜直接用石蜡包埋切片，这是因为钙和石蜡之间的密度不同，较难切除完整的切片。对含钙组织最好固定之后，再进行脱钙或二者同时进行，然后进行下游的实验，如脱水、透明、浸蜡、包埋、切片，用于脱钙的试剂很多，脱钙剂包括有机酸、无机酸、乙二胺四乙酸 (EDTA) 以及电解法脱钙。 在脱钙的过程中，应每隔一段时间检测一次脱钙程度，避免脱钙过度，脱钙过度会增加组织的损伤程度，进而影响染色结果。
本品由氨水、草酸铵等组成，当加入草酸铵后无沉淀产生，说明已达脱钙终点。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
组分表
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操作步骤 (仅供参考)
1、取正在使用的脱钙液 5ml，加入氨水溶液 1ml，充分混匀。
2、再加入草酸铵溶液 0.1ml，充分混匀。
3、观察有无沉淀，脱钙未完成时，有沉淀产生说明脱钙有效。当加入草酸铵后再无沉淀产生，说明已达脱钙终点。
注意事项
1、采用离子交换树脂法脱钙和 EDTA 法脱钙时，不宜采用本法。
2、每隔一段时间检测一次脱钙程度，避免脱钙过度，脱钙过度会增加组织的损伤程度，影响染色结果。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
有效期
12 个月有效。
When some tissues contain bone or calcified foci, calcium‑containing tissues should not be directly embedded in paraffin for sectioning. This is due to the density difference between calcium and paraffin, which makes it difficult to obtain intact sections. For calcium‑containing tissues, decalcification is preferably performed after fixation, or fixation and decalcification can be carried out simultaneously. Subsequent procedures such as dehydration, clearing, wax infiltration, embedding and sectioning are then performed. A variety of reagents are available for decalcification, including organic acids, inorganic acids, ethylenediaminetetraacetic acid (EDTA), and electrolytic decalcification. During decalcification, the degree of decalcification should be monitored at regular intervals to avoid over‑decalcification. Over‑decalcification will aggravate tissue damage and further affect staining results.
This product consists of ammonia solution, ammonium oxalate and other components. The absence of precipitate after adding ammonium oxalate indicates that the decalcification endpoint has been reached. This reagent is for research use only and is not intended for clinical diagnosis or other applications.
Component
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Procedure (For Reference Only)
1. Take 5 mL of the in‑use decalcifying solution, add 1 mL of ammonia solution, and mix thoroughly.
2. Add 0.1 mL of ammonium oxalate solution and mix thoroughly.
3. Observe for precipitate formation. If decalcification is incomplete, precipitate formation indicates effective decalcification. No precipitate after addition of ammonium oxalate indicates that the decalcification endpoint has been reached.
Notes
1. This method is not suitable for decalcification by ion‑exchange resin method or EDTA method.
2. Monitor the degree of decalcification at regular intervals to avoid over‑decalcification. Over‑decalcification will exacerbate tissue damage and affect staining results.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
Shelf‑life
Valid for 12 months.
| 组分货号 | 组分名称 | 物理外观 | 100T | 储存 |
| D1527424A | 氨水溶液 | 液体 | 100ml | RT |
| D1527424B | 草酸铵溶液 | 液体 | 10ml | RT |
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