储存缓冲液：10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
产品简介
本产品是由9条线状双链 DNA 片段组成，保存于1xLoading Buffer中，条带密集，需用高浓度胶低电压电泳，适用于较精确确认DNA片段大小。大小范围为 50-500bp，分别为 50bp、100bp、150bp、200bp、250bp、300bp、350bp、400bp、500bp；250 bp为加亮带，浓度为其他条带的2.5倍；5 ul产品中，每条带含量约40 ng，加亮带约100 ng。
使用建议
1. 建议点样量5 ul，宽胶孔适当增加上样量。
2. 建议用2.5-3.5 % Agarose，或5 %PAGE胶，电压4-10 v/cm，0.5xTBE缓冲液电泳。注意及时更换电泳缓冲液并使用新制备的凝胶，以达到理想的结果。
3. 通过EB进行染色，或者用其他的核酸染料进行染色，在紫外灯下观察电泳条带。
注意事项
1. 本产品已保存在1xLoading Buffer中，可直接进行电泳，使用方便，电泳图像清晰。
2. 使用含有EB的琼脂糖凝胶进行电泳检测时，将溴酚蓝条带电泳到约2/3的距离时即可，否则小片段部分由于EB脱离DNA，会导致条带变暗。
3. 随附 5xLoading buffer 可用于检测样品时使用。
5x Loading Buffer 成分：
10 mM Tris-HCl，5 mM EDTA，pH7.6，0.03 %溴酚蓝，0.03 %二甲苯青，30 % 甘油
组分表
| D745352 | Component | 100 T | 500T | Storage | | D745352A | DNA Ladder (50-500bp) | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. | | D745352B | 5xLoading buffer | 1 mL
| 5× 1 mL | -20℃. Avoid freeze/thaw cycle. |
|
Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
Product Introduction
This product is composed of 9 linear double stranded DNA fragments, stored in a 1xLoading Buffer, with dense bands that require high concentration gel and low voltage electrophoresis. It is suitable for accurately confirming the size of DNA fragments. The size range is 50-500bp, which are 50bp, 100bp, 150bp, 200bp, 250bp, 300bp, 350bp, 400bp, and 500bp respectively; 250 bp is the bright band, with a concentration 2.5 times that of other bands; In the 5 ul product, each strip contains about 40 ng, and the highlight strip contains about 100 ng.
Usage suggestions
1. It is recommended to sample 5 ul and increase the sample size appropriately for wide adhesive holes.
2. It is recommended to use 2.5-3.5% Agarose or 5% PAGE gel, voltage 4-10 v/cm, and 0.5xTBE buffer for electrophoresis. Pay attention to replacing the electrophoresis buffer in time and using the newly prepared gel to achieve ideal results.
3. Stain with EB or other nucleic acid dyes, and observe the electrophoretic bands under UV light.
matters needing attention
1. This product has been saved in a 1xLoading Buffer and can be directly used for electrophoresis. It is easy to use and the electrophoresis image is clear.
2. When using agarose gel containing EB for electrophoretic detection, the bromophenol blue band can be electrophoretic to about 2/3 of the distance, otherwise the small segment will become dark because EB is separated from DNA.
3. The accompanying 5xLoading buffer can be used for sample detection.
5x Loading Buffer components:
10 mM Tris-HCl，5 mM EDTA， pH7.6， 0.03% bromophenol blue, 0.03% xylene blue, 30% glycerol
Product component
| D745352 | Component | 100 T | 500T | Storage | | D745352A | DNA Ladder (50-500bp) | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. | | D745352B | 5xLoading buffer | 1 mL
| 5× 1 mL | -20℃. Avoid freeze/thaw cycle. |
|
ZJ25F0521827
危险品化学品经营许可证（带存储）