BioReagent,分子生物学级,即用型,用于核酸电泳,适用于电泳 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
储存缓冲液：GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
产品说明
1、本产品是由9条线状双链DNA片段组成,大小范围为100bp~5000bp,分别为100bp,250bp,500bp,750bp,1000bp,1500bp,2000bp,3000bp,5000bp。500bp和1500bp为加亮带指示,浓度为其它条带的2.5倍,便于电泳后观察。
2、5ul本产品中，常规条带的含量约为30ng，加亮带含量约75ng。
3、本产品已保存在 1xLoading Buffer 中，可直接用于电泳，使用方便。
4、本产品及配套的5xLoading buffer均已包含了Gelred核酸染料，配套使用，电泳后，可直接在紫外下观察，无需做后续染色处理。
5、本产品不适合于聚丙烯酰胺凝胶电泳。
6、推荐电泳缓冲液及琼脂糖凝胶浓度：
1xTAE电泳缓冲液
琼脂糖浓度：1.5%~2.0%
7、使用本产品体系，琼脂糖凝胶中无需加任何核酸染料。
使用说明
1、制备合适浓度的，不含任何核酸染料的琼脂糖凝胶。
2、凝胶浓度对DNA电泳的影响比较大，本品建议的琼脂糖凝胶浓度为1.5%~2.0%。
3、建议使用1xTAE缓冲液，电泳电压不超过10v/cm。
4、一般常见的3.5mm加样孔，DNA marker建议用量3~5ul，宽胶孔需适当增加样品量。
5、将待检测的样品与配套的5xLoading buffer，按照约4:1的比例混合，然后加入凝胶加样孔。
6、电泳至合适距离：
由于Gelred与DNA结合牢固，可以充分利用凝胶长度，电泳更长的距离，只要最小片段不要跑出凝胶即可，以有利于小片段电泳分离。一般溴酚蓝指示带距凝胶边缘不小于1cm。
7、电泳结束后，在紫外灯下观察电泳条带。
8、产品内所附的5x Loading buffer 用于与待检测样品混合后点样使用，含溴酚蓝和二甲苯青双指示剂。
9、如果是有大量可以直接点样的样品需要电泳检测，建议用Gelred制胶法来检测，不用预混样品，可以大量节约实验时间。
组分表
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Physical appearance: Liquid
Storage buffer solution: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
Product Description
1. This product is composed of 9 linear double stranded DNA fragments, ranging in size from 100bp to 5000bp, including 100bp, 250bp, 500bp, 750bp, 1000bp, 1500bp, 2000bp, 3000bp, and 5000bp. 500bp and 1500bp are highlighted bands with concentrations 2.5 times higher than other bands, making them easier to observe after electrophoresis.
2. In this product, the content of regular stripes is about 30ng, and the content of bright stripes is about 75ng.
3. This product has been saved in a 1xLoading Buffer and can be directly used for electrophoresis, making it easy to use.
4. This product and its accompanying 5xLoading buffer already contain Gelred nucleic acid dye, which can be used in conjunction. After electrophoresis, it can be directly observed under UV without the need for subsequent staining treatment.
5. This product is not suitable for polyacrylamide gel electrophoresis.
6. Recommended concentration of electrophoresis buffer and agarose gel:
1xTAE electrophoresis buffer
Agarose concentration: 1.5%~2.0%
7. Using this product system, no nucleic acid dye is needed in agarose gel.
instructions
1. Prepare agarose gel with appropriate concentration and without any nucleic acid dye.
2. The concentration of gel has a great influence on DNA electrophoresis. The recommended concentration of agarose gel for this product is 1.5%~2.0%.
3. It is recommended to use 1xTAE buffer with an electrophoresis voltage not exceeding 10v/cm.
4. The commonly used 3.5mm sample well requires a recommended amount of 3-5 μ l for DNA markers, and the sample size should be appropriately increased for wide gel wells.
5. Mix the sample to be tested with the matching 5xLoading buffer in a ratio of about 4:1, and then add it to the gel sampling hole.
6. Electrophoretic to appropriate distance:
Because Gelred is firmly bound to DNA, it can make full use of the length of gel, and the electrophoresis distance is longer, as long as the smallest segment does not run out of the gel, so as to facilitate the electrophoresis separation of small segments. Generally, the distance between the bromophenol blue indicator band and the edge of gel shall not be less than 1cm.
7. After electrophoresis, observe the electrophoresis bands under ultraviolet light.
8. The 5x loading buffer included in the product is used for sampling after mixing with the sample to be tested, and contains dual indicators of bromophenol blue and xylene blue.
9. If there are a large number of samples that can be directly sampled and require electrophoresis detection, it is recommended to use Gelred gel method for detection without pre mixing samples, which can save a lot of experimental time.
Product component
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Gelred-prestained DNA Ladder (100-5000bp) (R751622) - 1.5% agarose in TAE
Samples: Lysates at 5 µL per lane
Gelred-prestained DNA Ladder (100-5000bp) (R751622) is composed of 9 linear double stranded DNA fragments, ranging in size from 100bp to 5000bp, including 100bp, 250bp, 500bp, 750bp, 1000bp, 1500bp, 2000bp, 3000bp, and 5000bp. 500bp and 1500bp are highlighted bands with concentrations 2.5 times higher than other bands, making them easier to observe after electrophoresis.
| pH | 8.0-8.5 |
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0521828 |
分析证书 | R751622 |
| ZJ25F0521829 |
分析证书 | R751622 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Suitable for electrophoresis 级别指南 → 查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 → 查看 Suitable for molecular biology 级别指南 → 查看 for NA electrophoresis 级别指南 →
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