基本描述

别名

DNA Marker DL5000 | DNA电泳分子量标准R（100-5000bp) | DNA分子量标准 Marker (100-5000 bp)

英文别名

5kb DNA Ladder | 5kb DNA Marker | DNA Marker (100-5000 bp) | D5000 DNA Marker(100-5000bp) | DNA Marker DL5000

规格或纯度

BioReagent, 即用型, 适用于电泳, 适用于分子生物学, 用于核酸电泳, 100-5000bp, 9 bands, Blue, 100bp/ 250bp/ 500bp/ 750bp/ 1000bp/ 1500bp/ 2000bp/ 3000bp/ 5000bp

稳定性与储存

Store at room temperature short term (3-6 months). Store at 4℃ long term (24 months). Store at -20℃ long term (60 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

英文名称

DNA Ladder (100-5000bp)

储存温度

-20°C储存,避免反复冻融

运输条件

超低温冰袋运输

产品介绍

产品说明

储存缓冲液：10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6

1. 本产品是由9条线状双链DNA片段组成，大小范围为100bp~5000bp，分别为100bp、250bp、500bp、750bp、1000bp、1500bp、2000bp、3000bp、5000bp。500bp和1500bp为加亮带指示，浓度为其它条带的2.5倍，便于电泳后观察。
2. 5ul本产品中,常规条带的含量约为30ng,加亮带含量约75ng。
3. 本产品已保存在1xLoading Buffer 中,可直接用于电泳,使用方便。
4. 本产品不能用于聚丙烯酰胺凝胶电泳。
推荐电泳缓冲液及琼脂糖凝胶浓度:
本产品推荐使用1xTAE电泳缓冲液，推荐琼脂糖浓度：1.5%~2.0%，小片段电泳，建议使用GelRed核酸染料。
使用说明
1. 制备含核酸染料的琼脂糖凝胶，如EB或者GelRed。
2. 凝胶浓度对DNA电泳的影响比较大，本品建议的琼脂糖凝胶浓度为1.5%~2.0%。
3. 建议使用1xTAE缓冲液，电泳电压不超过10v/cm。
4. 一般常见的3.5mm加样孔，建议用量3~5ul,宽胶孔适当增加上样量。
5. 电泳至合适距离：对于EB，Goldview，GRBlue等核酸染料，一般电泳时溴酚蓝条带的位置不要超过凝胶三分之二，否则，小片段会由于核酸染料与DNA脱离而变弱。对于GelRed等核酸染料，可以电泳更长的距离，只要最小片段不要跑出凝胶即可。一般溴酚蓝指示带距凝胶边缘不小于1cm。
6. 电泳结束后，在紫外灯下观察电泳条带。
7. 产品内所附的5x Loading buffer 用于与待检测样品混合后点样使用，含溴酚蓝和二甲苯青双指示剂。

组分表

D745354	Component	100 T	500T	Storage
D745354A	DNA Ladder (100-5000bp)	500 μL	5× 500 μL	-20℃. Avoid freeze/thaw cycle.
D745354B	5xLoading buffer	1mL	5×1 mL	-20℃. Avoid freeze/thaw cycle.

Product Description:

1. This product consists of 9 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 5000 bp, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp and 5000 bp. The 500 bp and 1500 bp bands are highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.

2. In 5 ul of this product, the content of the regular bands is approximately 30 ng, while the highlighted band contains about 75 ng.

3. The product is preserved in 1x Loading Buffer and can be directly used for electrophoresis, making it convenient to use.

4. This product is not suitable for polyacrylamide gel electrophoresis.

Recommended Electrophoresis Buffer and Agarose Gel Concentration:

This product is recommended to be used with 1x TAE electrophoresis buffer, with a suggested agarose concentration of 1.5% to 2.0%. For electrophoresis of smaller fragments, it is recommended to use GelRed nucleic acid dye.

Usage Instructions:

1. Prepare an agarose gel containing a nucleic acid dye, such as EB or GelRed.

2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.5% to 2.0%.

3. It is suggested to use 1x TAE buffer, with an electrophoresis voltage not exceeding 10 v/cm.

4. For common 3.5 mm sample wells, a loading volume of 3 to 5 ul is recommended, with an appropriate increase for wider gel wells.

5. Run the electrophoresis to the appropriate distance: For nucleic acid dyes such as EB, Goldview, and GRBlue, the bromophenol blue front should not exceed two-thirds of the gel, otherwise, smaller fragments may weaken due to the detachment of the nucleic acid dye from DNA. For dyes like GelRed, longer distances can be used as long as the smallest fragment does not run out of the gel. Generally, the bromophenol blue indicator band should be at least 1 cm away from the edge of the gel.

6. After electrophoresis, observe the bands under a UV lamp.

7. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, containing both bromophenol blue and xylene cyanol as indicators.

Product component

D745354	Component	100 T	500T	Storage
D745354A	DNA Ladder (100-5000bp)	500 μL	5× 500 μL	-20℃. Avoid freeze/thaw cycle.
D745354B	5xLoading buffer	1mL	5×1 mL	-20℃. Avoid freeze/thaw cycle.

产品属性

pH	8.0-8.5

图片

1.5% Agarose in TAE: DNA Ladder (100-5000bp) (D745354)

DNA Ladder (100-5000bp) (D745354) - 1.5% Agarose in TAE
Samples: Lysates at 5 µL per lane
This product consists of 9 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 5000 bp, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp and 5000 bp. The 500 bp and 1500 bp bands are highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.