储存缓冲液：GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
产品说明:
1、本产品是由13条线状双链DNA片段组成,大小范围为250bp-12kb,分别为250bp, 500bp, 750bp, 1000bp, 1500bp, 2000bp, 2500bp, 3000bp, 4000bp, 5000bp, 6000bp, 8000bp, 12000bp. 1500bp和4000bp为加亮带指示,浓度为其它条带的2.5倍,便于电泳后观察。
2、5ul本产品中，常规条带的含量约为30ng，加亮带含量约75ng。本产品已保存在 1xLoading Buffer 中，可直接用于电泳，使用方便。
3、本产品及配套的5xLoading buffer均已包含了Gelred核酸染料，配套使用，电泳后，可直接在紫外下观察，无需做后续染色处理。
4、本产品不适合于聚丙烯酰胺凝胶电泳。
5、推荐电泳缓冲液及琼脂糖凝胶浓度：
6、本产品推荐使用1xTAE电泳缓冲液，推荐琼脂糖浓度：1.0%~1.5%，使用本产品体系，琼脂糖凝胶中无需加任何核酸染料。
使用说明：
1、制备合适浓度的，不含任何核酸染料的琼脂糖凝胶。
2、凝胶浓度对DNA电泳的影响比较大，本品建议的琼脂糖凝胶浓度为1.0%~1.5%。
3、建议使用1xTAE缓冲液，电泳电压不超过10v/cm。
4、一般常见的3.5mm加样孔，DNA marker建议用量3~5ul，宽胶孔需适当增加样品量。
5、将待检测的样品与配套的5xLoading buffer，按照约4:1的比例混合，然后加入凝胶加样孔。
6、电泳至合适距离：
由于Gelred与DNA结合牢固，可以充分利用凝胶长度，电泳更长的距离，只要最小片段不要跑出凝胶即可，以有利于小片段电泳分离。一般溴酚蓝指示带距凝胶边缘不小于1cm。
7、电泳结束后，在紫外灯下观察电泳条带。
8、产品内所附的5x Loading buffer 用于与待检测样品混合后点样使用，含溴酚蓝和二甲苯青双指示剂。
9、如果是有大量可以直接点样的样品需要电泳检测，建议用Gelred制胶法来检测，不用预混样品，可以大量节约实验时间。
组分表
| R751625 | Component | 100 T | 500T | Storage | | R751625A | Gelred-prestained DNA Ladder (250-12000bp) | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. | | R751625B | Gelred-prestained 5xLoading buffer | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. |
|
Product Description:
1. This product consists of thirteen linear double-stranded DNA fragments with a size range of 250bp to 12kb, specifically 250bp, 500bp, 750bp, 1000bp, 1500bp, 2000bp, 2500bp, 3000bp, 4000bp, 5000bp, 6000bp, 8000bp, and 12000bp. The 1500bp and 4000bp bands serve as intensified indicator bands, with a concentration 2.5 times higher than that of the other bands, facilitating observation after electrophoresis.
2. In 5μl of this product, the content of the regular bands is approximately 30ng, while the content of the intensified band is about 75ng. This product is already preserved in a 1x Loading Buffer and can be directly used for electrophoresis, offering convenience in use.
3. Both this product and the accompanying 5x Loading Buffer contain the Gelred nucleic acid stain. When used together, after electrophoresis, the bands can be directly observed under ultraviolet light without the need for subsequent staining procedures.
4. This product is not suitable for polyacrylamide gel electrophoresis.
Recommended Electrophoresis Buffer and Agarose Gel Concentration:
It is recommended to use 1x TAE electrophoresis buffer for this product, with a recommended agarose concentration of 1.0% to 1.5%. When using this product system, no additional nucleic acid stains are required in the agarose gel.
Usage Instructions:
1. Prepare an agarose gel of the appropriate concentration without any nucleic acid stains.
2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.0% to 1.5%.
3. It is suggested to use 1x TAE buffer for electrophoresis, with a voltage not exceeding 10v/cm.
4. For common 3.5mm sample wells, the recommended volume of DNA marker is 3 to 5μl. For wider gel wells, the sample volume should be appropriately increased.
5. Mix the samples to be tested with the accompanying 5x Loading Buffer at a ratio of approximately 4:1, and then load into the gel sample wells.
6. Run the electrophoresis to an appropriate distance:
Since Gelred binds firmly to DNA, it is possible to fully utilize the length of the gel and run a longer distance, as long as the smallest fragment does not run out of the gel, which is beneficial for the separation of small fragments. Generally, the bromophenol blue indicator band should be no less than 1cm away from the edge of the gel.
7. After electrophoresis, observe the electrophoresis bands under a UV lamp.
8. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, and it contains both bromophenol blue and xylene cyan FF as dual indicators.
9. If there are a large number of samples that can be directly loaded for electrophoresis testing, it is recommended to use the Gelred gel method for detection, without pre-mixing the samples, which can greatly save experimental time.
Product component
| R751625 | Component | 100 T | 500T | Storage | | R751625A | Gelred-prestained DNA Ladder (250-12000bp) | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. | | R751625B | Gelred-prestained 5xLoading buffer | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. |
|
ZJ25F0521824
危险品化学品经营许可证（带存储）