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超氧化物歧化酶 2 , 线粒体( SOD2 ), 又称锰依赖性超氧化物歧化酶(MnSOD),是一种酶,是由 6 号染色体上的 SOD2 基因编码的。该基因的替代剪接导致多种转录变体。该基因是铁/锰超氧化物歧化酶家族的成员。它编码一种线粒体蛋白,形成一个同源四聚体,每个亚单位结合一个锰离子。 该蛋白与氧化性磷酸化的超氧化物副产品结合,并将其转化为过氧化氢和二原子氧。SOD2 基因包含五个外显子,被四个内含子打断,一个非典型的 5′-近端启动子,拥有一个富含 GC 的区域来代替 TATA 或 CAAT,第二个内含子中还有一个增强子。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠锰超氧化物歧化酶(Mn-SOD;SOD2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠锰超氧化物歧化酶(Mn-SOD;SOD2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Mn-SOD;SOD2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Mn-SOD;SOD2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.