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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
IL-17A 是一种蛋白质,由 IL17A 基因编码。在啮齿动物中,IL-17A 曾经被 称为 CTLA8,因为它与一个病毒基因(O40633)相似。它是一种由活化的 T 细胞产生的促炎症细胞因子。该细胞因子可调节 NF-kappaB 和丝裂原活 化蛋白激酶的活性。该细胞因子可刺激 IL6 和环氧化酶-2(PTGS2/COX-2) 的表达,并增强一氧化氮(NO)的产生。它的高水平与一些慢性炎症性疾 病有关,包括类风湿性关节炎、银屑病和多发性硬化症。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白细胞介素17A(IL-17A)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素17A(IL-17A)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-17A. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-17A present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.