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胞外超氧化物歧化酶(SOD3),也被称为超氧化物歧化酶B,是由SOD3基因编码的酶。该基因编码超氧化物歧化酶蛋白家族的成员。SOD是一种抗氧化剂酶,可催化两个超氧化物自由基分解为过氧化氢和氧气。该基因的产物被认为可以保护脑,肺和其他组织免受氧化应激。该蛋白质分泌到细胞外空间并形成糖基化的同源四聚体,通过与硫酸乙酰肝素蛋白多糖和胶原蛋白的相互作用锚定到细胞外基质 (ECM) 和细胞表面。一部分蛋白质在分泌之前在C端附近被切割,以产生不与ECM相互作用的循环四聚体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人胞外超氧化物歧化酶(SOD3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人胞外超氧化物歧化酶(SOD3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SOD3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SOD3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.