计算溶液所需的质量、体积或浓度。
BioReagent,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
固定的目的在于保存细胞和组织的原有形态结构,固定剂能阻止内源性溶酶体酶对自身组织和细胞的自溶、抑制细菌和霉菌的生长。固定剂通过凝固、生成添加化合物等使蛋白质内部结构发生改变,从而使酶失活。固定液主要分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐类固定液等,较常用的是醛类中的福尔马林、醇类中的乙醇。
FPA 固定液 (FPA Fluid) 主要由福尔马林、乙醇、乙酸混合而成,用于固定一般的植物材料,24h 固定后效果比 FAA 固定液更好,并可长期保存材料。
操作步骤(仅供参考):
1、一般需固定 24h。
2、固定后可长期保存。
注意事项:
1、FPA固定液对人体有一定的损害,请在通风好的环境下小心操作,避免吸入。
2、组织取材的厚度不同,固定时间也不同,对组织恰当的选材有利于固定液的渗透。常规活检组织比较适合的厚度为 2~4mm,一般不超过 6mm。
3、固定液的容量应足够,一般固定液与组织块的体积比率应大于 10:1。如果容积不够大,可以在固定期间更换 1~3 次固定液。
4、温度对固定的影响很明显,提高温度可以加速固定作用,但温度不宜过高。
5、取出新鲜组织后,应及时固定,无法及时固定时,应保存于生理盐水中及时送检。
6、为了您的安全和健康,请穿实验服并戴一次性手套操作。
7、本产品仅用于科研,严禁他用。
The purpose of fixation is to preserve the original morphology and structure of cells and tissues. Fixatives prevent autolysis of tissues and cells by endogenous lysosomal enzymes and inhibit the growth of bacteria and molds. Fixatives alter the internal structure of proteins through coagulation, formation of additive compounds, and other mechanisms, thereby inactivating enzymes. Fixative solutions are mainly classified into aldehyde fixatives, mercuric fixatives, alcoholic fixatives, oxidizing fixatives, picrate fixatives, etc. Among the commonly used ones are formalin from aldehydes and ethanol from alcohols.
FPA Fixative Solution (FPA Fluid) is mainly composed of formalin, ethanol and acetic acid. It is used for fixing general plant specimens. Fixation for 24 hours yields better results than FAA fixative and allows long‑term preservation of specimens.
Procedure (For Reference Only):
1. Fixation generally requires 24 hours.
2. Specimens can be stored long‑term after fixation.
Precautions:
1. FPA fixative is harmful to the human body. Please handle with care in a well‑ventilated environment and avoid inhalation.
2. Fixation time varies with the thickness of tissue samples. Proper tissue preparation facilitates penetration of the fixative. The suitable thickness for routine biopsy tissues is 2–4 mm, generally not exceeding 6 mm.
3. The volume of fixative should be sufficient; the volume ratio of fixative to tissue block should generally be greater than 10:1. If the volume is inadequate, the fixative may be replaced 1–3 times during fixation.
4. Temperature significantly affects fixation. Increasing temperature accelerates fixation, but the temperature should not be excessively high.
5. Fresh tissues should be fixed promptly after collection. If immediate fixation is not possible, tissues should be stored in physiological saline and submitted for testing without delay.
6. For your safety and health, please wear a lab coat and disposable gloves during operation.
7. This product is for research use only and strictly prohibited for other purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | F1520383 |