计算溶液所需的质量、体积或浓度。
BioReagent,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
固定液主要分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐固定液等,较为常用的是醛类中的福尔马林、醇类中的乙醇。
Clarke固定液 (Clarke Fixative Solution) 又称Clarke溶液,主要乙醇、乙酸混合而成,固定后对于HE染色有较好的组织学形态。其优点是可以保护核酸,其缺点是1、易脱去油脂,因此不适宜脂肪染色的固定。2、适用于短期固定,并且固定后应将组织移到95%乙醇中。
操作步骤(仅供参考):
1、按实验具体要求操作。
2、一般仅需固定30~60min。
3、如果组织块大,可适当延长固定时间,但不宜超过24h。固定后,应将组织移到95%乙醇中。
注意事项:
1、组织取材的厚度不同,固定时间也不同,对组织恰当的选材有利于固定液的渗透。
2、固定液的容量应足够,一般固定液与组织块的体积比率应大于10:1。如果容积不够大,可以在固定期间更换1~3次固定液。
3、取出新鲜组织后,应及时固定,无法及时固定时,应保存于生理盐水中及时送检。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
5、本产品仅用于科研,严禁他用。
Fixative solutions are mainly classified into aldehyde fixatives, mercuric fixatives, alcoholic fixatives, oxidizing fixatives, picrate fixatives, etc. Among the commonly used ones are formalin from aldehydes and ethanol from alcohols.
Clarke Fixative Solution, also known as Clarke’s solution, is mainly composed of ethanol and acetic acid. It provides favorable histological morphology for HE staining after fixation. Its advantage is that it can preserve nucleic acids. Its disadvantages are as follows:
It tends to remove lipids, so it is not suitable for fixation in fat staining.
It is only suitable for short‑term fixation, and tissues should be transferred to 95% ethanol after fixation.
Procedure (For Reference Only):
1. Perform the procedure according to specific experimental requirements.
2. Generally, fixation takes only 30–60 minutes.
3. For larger tissue blocks, the fixation time may be appropriately extended, but should not exceed 24 hours. After fixation, the tissue should be transferred to 95% ethanol.
Precautions:
1. Fixation time varies with the thickness of the tissue sample. Proper tissue selection facilitates penetration of the fixative.
2. The volume of fixative should be sufficient; generally, the volume ratio of fixative to tissue block should be greater than 10:1. If the volume is inadequate, the fixative may be replaced 1–3 times during fixation.
3. Fresh tissues should be fixed promptly after excision. If immediate fixation is not possible, they should be stored in physiological saline and submitted for testing without delay.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
5. This product is for research use only and strictly prohibited for other purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1520388 | |
| 分析证书 | C1520388 |