计算溶液所需的质量、体积或浓度。
BioReagent,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Carnoy 固定液(Carnoy's Fluid)又称卡诺氏固定液,能很好地显示 DNA 和 RNA,也常用于糖原和尼氏体的固定,有极快的渗透力,固定最多不超 24h。其特点是能迅速穿透细胞,将其固定并维持染色体结构的完整性,还能够增强染色体的嗜碱性,达到优良染色效果。推荐用于 RNA 和 DNA 染色的组织固定,亦可用于糖原染色的组织固定,能够使糖原呈细微颗粒状。由于该液穿透力强,亦可固定外膜致密不宜渗透的组织。组织固定后暂时不用,可置于 70%乙醇中保存。
保存:10~30℃,避光,12 个月。
使用方法
1、一般小块组织固定 30~40min。
2、稍大块组织 2~4h。
3、如果组织块大,可适当延长固定时间,但不宜超过 24h。
注意事项
1、Carnoy 固定液对人体有一定的损害,请小心操作。不宜用于固定脂类染色的组织。
2、组织取材的厚度不同,固定时间也不同。
3、固定液的容量应足够,一般固定液与组织块的体积比率应大于 10:1。
4、温度对固定的影响很明显,提高温度可以加速固定作用,但温度不宜过高。
5、取出新鲜组织后,应及时固定,无法及时固定时,应保存于生理盐水中及时送检。
6、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Carnoy's Fluid is widely used for the visualization of DNA and RNA, as well as the fixation of glycogen and Nissl bodies. It features extremely rapid penetration, and the fixation duration shall not exceed 24 hours. This fixative can quickly infiltrate cells to preserve the integrity of chromosome structure, and enhance the basophilia of chromosomes for optimal staining results. It is recommended for tissue fixation prior to RNA, DNA and glycogen staining, and can render glycogen into fine granular forms. Thanks to its strong penetrability, it is also suitable for tissues with dense outer membranes that are difficult for common fixatives to infiltrate. Fixed tissues can be temporarily stored in 70% ethanol if not used immediately.
Instructions for Use:
1. Fix small tissue pieces for 30 to 40 minutes.
2. Fix medium-sized tissue pieces for 2 to 4 hours.
3. Prolong the fixation time appropriately for large tissue blocks, but do not exceed 24 hours.
Precautions:
1. Carnoy's Fluid is harmful to the human body. Handle with caution. It is not suitable for tissues intended for lipid staining.
2. Fixation time varies depending on the thickness of tissue specimens.
3. Use a sufficient volume of fixative. The volume ratio of fixative to tissue block should be greater than 10:1.
4. Temperature exerts a notable effect on fixation. Elevating temperature accelerates the fixation process, yet excessive temperature should be avoided.
5. Fresh tissues must be fixed promptly. If immediate fixation is not available, preserve the tissues in normal saline and deliver for processing in a timely manner.
6. Wear a lab coat and disposable gloves during operation for personal safety and health protection.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1373509 | |
| 分析证书 | C1373509 | |
| 分析证书 | C1373509 | |
| 分析证书 | C1373509 | |
| 分析证书 | C1373509 |