计算溶液所需的质量、体积或浓度。
BioReagent,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
固定的目的在于保存细胞和组织的原有形态结构,固定剂能阻止内源性溶酶体酶对自身组织和细胞的自溶、抑制细菌和霉菌的生长。固定剂通过凝固、生成添加化合物等使蛋白质内部结构发生改变,从而使酶失活。固定剂对细胞核细胞外成分发生物理改变。固定液主要分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐类固定液等,较为常用的是醛类中的福尔马林、醇类中的乙醇。
甲醛钙固定液 (10%) 主要由甲醛、钙盐、去离子水组成,该固定液适合于绝大多数组织的固定。
操作步骤(仅供参考):
1、按实验具体要求操作。一般标本固定时间控制在1~4h/mm,大标本应适当延长固定时间。
2、固定好的组织,可在甲醛钙固定液 (10%) 或70%乙醇中长期保存。
注意事项:
1、甲醛钙固定液 (10%) 有一定刺激性和腐蚀性,请在通风较好的环境下操作,避免吸入。
2、固定液pH最好接近用中性,pH范围6~8。
3、温度对固定的影响很明显,提高温度可以加速固定作用,但温度不宜过高。
4、取出新鲜组织后,应及时固定,无法及时固定时,应保存于生理盐水中及时送检。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
6、本产品仅用于科研,严禁他用。
The purpose of fixation is to preserve the original morphological structure of cells and tissues. Fixatives can prevent autolysis of tissues and cells by endogenous lysosomal enzymes and inhibit the growth of bacteria and molds. Fixatives alter the internal structure of proteins through coagulation, formation of adducts, and other mechanisms, thereby inactivating enzymes. Fixatives also induce physical changes in the nucleus and extracellular components of cells. Fixatives are mainly classified into aldehyde fixatives, mercuric fixatives, alcoholic fixatives, oxidizing fixatives, picrate fixatives, etc. Among the commonly used ones are formalin in the aldehyde group and ethanol in the alcoholic group.
Formaldehyde Calcium Fixative (10%) is mainly composed of formaldehyde, calcium salts, and deionized water. This fixative is suitable for the fixation of most tissues.
Procedure (For Reference Only):
1. Operate according to the specific requirements of the experiment. Generally, the fixation time is controlled at 1–4 hours per mm of specimen thickness; the fixation time should be appropriately prolonged for large specimens.
2. Fixed tissues can be stored long-term in 10% Formal-Calcium Fixative or 70% ethanol.
Precautions:
1. 10% Formal-Calcium Fixative is somewhat irritating and corrosive. Please operate in a well-ventilated environment and avoid inhalation.
2. The pH of the fixative should preferably be close to neutral, within the range of 6–8.
3. Temperature has a significant effect on fixation: increasing temperature accelerates fixation, but the temperature should not be excessively high.
4. Fresh tissues should be fixed promptly after excision. If fixation cannot be performed immediately, the tissues should be preserved in physiological saline and submitted for testing without delay.
5. For your safety and health, please wear a laboratory coat and disposable gloves during operation.
6. This product is for research use only and strictly prohibited for other purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | F1520402 |