计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
GDH(EC 1.4.1.2)广泛分布于植物中,和谷氨酸合成酶(GOGAT)共同参与谷氨酸的合成,在氨同化和转化成有机氮化合物的代谢中起重要作用。 测定原理:GDH催化NH₄⁺、α-酮戊二酸和NADH,生成谷氨酸和NAD⁺,引起340nm吸光度下降。通过测定340nm吸光度的下降速率,计算GDH活性。
Glutamate Dehydrogenase (EC 1.4.1.2) is widely distributed in plants. Together with Glutamine Synthase (GOGAT), it participates in the synthesis of glutamate and plays a crucial role in the metabolism of ammonia assimilation and its conversion into organic nitrogen compounds. Assay Principle: GDH catalyzes the reaction of NH₄⁺, α-ketoglutarate, and NADH to produce glutamate and NAD⁺, resulting in a decrease in absorbance at 340 nm. The activity of GDH is calculated by measuring the rate of decrease in absorbance at 340 nm.