计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,生物活性,重组,高性能 级 提供 ≥90%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α-1,4-半乳糖基转移酶(LgtC),EC 2.4.1.228。酶基因来源于Neisseria meningitides。 可用于将半乳糖转移到 以Gal为非还原端的糖链上。本产品利用大肠杆菌重组表达,纯度高,活性高
反应式:
UDP-Gal + β1-4GalβGlcOR —— α1-4Galβ1-4GalβGlcOR + UDP
使用方法
1.按照反应体系加入底物,5mM MgCl₂ 1mM DTT,可适当加入缓冲盐(如:Tris-HCl),调节pH为7.5,加入适量LgtC(根据反应需求);
2.混匀后,将样品置于 37℃孵育2-4h
【注】体系放大时候,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
【注】多数常见的生物缓冲液适用,比如 Tris 或 PBS,不在此 pH 范围的缓冲液(如醋酸缓冲液)可能适用,但是孵育时间或酶量需要根据实际情况进行优化。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
α-1,4-Galactosyltransferase (LgtC), EC 2.4.1.228.The enzyme gene is derived from Neisseria meningitidis. It catalyzes the transfer of galactose onto glycan chains with galactose at the non‑reducing end. This product is recombinantly expressed in Escherichia coli, featuring high purity and high activity.
Reaction Equation
UDP‑Gal + β1‑4GalβGlcOR → α1‑4Galβ1‑4GalβGlcOR + UDP
Usage Instructions
Add substrates to the reaction system, along with 5 mM MgCl₂ and 1 mM DTT. Appropriate buffer salts (e.g., Tris‑HCl) may be supplemented to adjust the pH to 7.5, followed by addition of an appropriate amount of LgtC according to reaction requirements.
After mixing, incubate the sample at 37 °C for 2–4 hours.
Notes
For scaled‑up reactions, incubation time or enzyme dosage should be adjusted accordingly; low‑speed shaking is recommended for mixing.
Most common biological buffers (e.g., Tris or PBS) are suitable. Buffers outside this pH range (e.g., acetate buffer) may also be applicable, yet incubation time or enzyme dosage needs optimization based on practical conditions.
Precautions
For your safety and health, wear a lab coat and disposable gloves during operation. This product is for research use only.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | G293642 | |
| 分析证书 | G293642 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 High Performance 级别指南 → 查看 EnzymoPure™ 级别指南 →