BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乳酸脱氢酶(lactate dehydrogenase，LDH或LD)属于氧化还原酶，能够催化氢氧原子或电子从一种底物转移到另一种底物上乳酸脱氢酶是糖酵解和糖异生的一个极其重要的酶，含有锌离子，广泛分布于人和动物组织、植物和微生物中，能可逆的催化乳酸(L)和丙酮酸(P)之间的氧化还原反应；其反应公式：乳酸+NAD⁺↔丙酮酸+NADH+H⁺，其中：L→P为正向反应；P→L为逆向反应。 该试剂盒检测原理是以NAD为受氢体，乳酸脱氢酶催化乳酸脱氢生成丙酮酸，丙酮酸与二硝基苯肼反应生成丙酮酸二硝基苯腙，后者在碱性溶液中呈棕红色，颜色深浅与丙酮酸浓度呈正比，分光光度计检测440nm处吸光度，通过测得的丙酮酸含量计算酶的活性。该方法的优点是：1、试剂原料易获得；2、较为经典的方法；3、适用于手工操作。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
Lactate Dehydrogenase (LDH or LD) is an oxidoreductase enzyme that catalyzes the transfer of hydrogen/oxygen atoms or electrons from one substrate to another. It is an extremely important enzyme in glycolysis and gluconeogenesis, contains zinc ions, and is widely distributed in human and animal tissues, plants, and microorganisms. It reversibly catalyzes the oxidation-reduction reaction between lactate (L) and pyruvate (P); the reaction formula is: Lactate + NAD⁺ ↔ Pyruvate + NADH + H⁺, where the L→P reaction is the forward reaction and the P→L reaction is the reverse reaction. The detection principle of this kit is as follows: Using NAD as a hydrogen acceptor, lactate dehydrogenase catalyzes the dehydrogenation of lactate to form pyruvate. The pyruvate then reacts with dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which appears reddish-brown in an alkaline solution. The color intensity is proportional to the pyruvate concentration. The absorbance is measured at 440 nm using a spectrophotometer, and the enzyme activity is calculated based on the measured pyruvate content. The advantages of this method are: 1. The raw materials for reagents are easily obtained; 2. It is a relatively classic method; 3. It is suitable for manual operation. This kit is intended for research use only and is not for use in diagnostic procedures.
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