BIOMATERIALS
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2025
·
IF 12.9
Bikun Zhou,Xingan Qiu,Pengzhen Bu,Xuezhe Liu,Xinqiang Hu,Yongsheng Yu,Ning Wang,Jian Dong,Qian Feng,Kaiyong Cai
Photodynamic therapy achieves antibacterial effects by generating reactive oxygen species (ROS), and is therefore used in the clinical treatment of infectious wounds. However, generated ROS also causes damage to surrounding normal tissues and exacerbates the inflammatory response inevitably, which restrict the application scope of PDT. To address this problem, we developed a new photosensitizer, L-arginine(L-Arg) modified zinc oxide (ZnO) nanoparticles (AL-ZnO NPs). Under sunlight illumination, the high-dose oxygen (·O 2 - ) and hydroxyl radicals (·OH) generated by ZnO were converted to low-dose yet highly reactive peroxynitrite (ONOO - ) through the L-Arg mediated cascade reaction, which preserved antibacterial activity of produced ROS while preventing the adverse effects of excessive ROS leakage on surrounding tissues. Concurrently, sustained Zn 2+ releasing from AL-ZnO NPs activated intracellular superoxide dismutase (SOD) associated antioxidant pathway to counteract ROS-induced negative effect to normal cells, while promoting macrophage polarization from M1 to M2 to reduce inflammation. Then, the well-designed AL-ZnO NPs were encapsulated freeze-thaw polyvinyl alcohol (PVA) hydrogel to prepare composite hydrogel dressing. Our in vitro and in vivo evaluations confirmed that this hydrogel addressed the challenge caused by excessive ROS in PDT, achieved potent antibacterial efficacy, protected normal tissue from ROS damaging, reduced inflammation, and finally promoted the healing of infected wounds.
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Agronomy-Basel
·
2025
·Vol. 15
·期 11
·2483
·
IF 3.4
Meijun Liu,Liangzhu Gong,An Yan,Wenjing Liu,Haojie Li,Peiyi Guo
Tris(2-chloroethyl) phosphate (TCEP), as an organophosphate contaminant, poses a significant threat to the growth and development of plants, especially roots. This study aimed to clarify the mechanisms of TCEP’s toxicity and damage to root systems, as well as the mechanisms of its damage to the respiration and energy metabolism of alfalfa root cells. The results showed that TCEP obviously affected the root length, root surface area, root volume, and root diameter of alfalfa. With increasing stress intensity, the total mitochondrial respiration rate and Cytochrome C Oxidase (COX) pathway respiration rate progressively declined, while the Alternative Oxidase (AOX) pathway respiration rate and its proportion of total respiration gradually rose. In addition, adenosine triphosphate (ATP) content and root vigor were significantly reduced. Moreover, with an increase in TCEP concentration, root superoxide anion radical content in alfalfa root cells was significantly elevated, while superoxide dismutase (SOD) and catalase (CAT) activities were significantly lowered, and ascorbate peroxidase (APX) and peroxidase (POD) activities were significantly enhanced. The present study indicated that respiration was disrupted, causing a lack of ATP in root cells under TCEP. Both the overproduction of reactive oxygen species (ROS) from the mitochondrial respiratory electron transport chain (mECT) and the deficiency of ROS-scavenging enzymes caused ROS accumulation, which led to the destruction of the cell membrane structure and exacerbated the disruption of the respiratory metabolism. The disruption of the conversion and reuse of energy by TCEP affected root growth and development.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Meiyun Shang,Juan Hu,Mengjia Shi,Yunpeng Fan,Yuan Zou,Yong Wang,Jinhong Guo,Cuiping Mao
Anti-cyclic citrullinated peptide (anti-CCP) antibodies are key biomarkers for rheumatoid arthritis and vital for its early diagnosis and monitoring. Conventional chemiluminescence immunoassays (CLIA) rely on streptavidin-functionalized magnetic beads (SA-MBs), which suffer from limited binding capacity and require labor-intensive chemical conjugation steps. Here, we report a MBs carrier modified with zirconium (Zr)-based metal-organic frameworks (MB@MOF), enabling rapid and efficient SA functionalization via one-step physicochemical adsorption. Compared to the conventional EDC/NHS method, our approach shortens the modification time by at least sixfold and eliminates the need for chemical crosslinkers. The MB@MOF carrier was constructed by anchoring Zr-MOF nanoparticles, synthesized with TCPE as the organic ligand, onto MBs. This carrier supports stable and dense SA loading while preserving native SA activity, thereby allowing highly sensitive detect anti-CCP antibodies. The developed MB@MOF carrier-based CLIA method achieved a detection limit of 0.1 RU/mL and a linear range of 0.5–200 RU/mL. Clinical validation using 36 clinical serum samples showed strong agreement with results from standard clinical analyzers, achieving an overall diagnostic accuracy of 91.7 %. This strategy significantly simplifies the assay workflow and holds strong potential for high-performance and rapid diagnostics of rheumatoid arthritis in clinical application.
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JOURNAL OF CATALYSIS
·
2025
·
IF 6.5
Wenli Zhang,Xu Han,Xianhong Zheng,Yi Yang,Zhiqi Zhao,Haiwei Yang,Yi Zhang,Chengbo Du,Zongqian Wang,Qingbo Xu,Changlong Li,Peng Wang
Solar-driven photocatalysis is inherently limited by its diurnal discontinuity. Herein, we report a V 2 O 5 /g-C 3 N 4 /Ti 3 C 2 triadic composite that transcends this limitation via a “micro proton-battery-like” mechanism, enabling round-the-clock catalytic activity. The optimized composite achieves 93.9 % tetracycline degradation under visible light and, remarkably, maintains 64.1 % efficiency in the dark. This is underpinned by an 8-fold higher electron storage capacity (0.16 μmol/mg) than pristine g-C 3 N 4 , achieved via reversible V 5+ /V 4+ redox transitions. DFT calculations corroborate charge localization in V 3d orbitals during the light-driven “charging” phase and subsequent Ti 3 C 2 -mediated electron release for ·O 2 − generation in the dark. This work establishes a groundbreaking paradigm that unifies photocatalysis with electrochemical energy storage for persistent environmental remediation.
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ANIMAL REPRODUCTION SCIENCE
·
2025
·
IF 3.3
Xi Huang,Zhangshan Gao,Peichao Gao,Helong Ji,Laizhi Xu,Dengfei Feng,Wentao Fan,Suquan Song
Stress is a well-established disruptor of reproductive function in poultry. Gamma-aminobutyric acid (GABA), a notable postbiotic derived from Lactobacillus plantarum ( L. plantarum ), has attracted increasing attention as a dietary supplement. While existing research predominantly focuses on GABA applications in broilers and other species, its effects on laying hens remain underexplored. This study investigated whether GABA and L. plantarum 1-2-3 supplementation mitigated corticosterone (CORT)-induced chronic stress in laying hens. Results demonstrated that both GABA and L. plantarum 1-2-3 effectively mitigated CORT-induced declines in laying performance and ovarian damage. They reduced ovarian oxidative stress, inflammation, and atretic follicles by enhancing antioxidant capacity and normalizing expression of inflammatory factors ( IL18 , LITAF , IL6 , IL1B) , and apoptosis-related factors ( BCL2 , BAX , CASP9 , CASP3 ). Furthermore, GABA and L. plantarum 1-2-3 markedly increased the serum reproductive hormone levels, as well as the transcription level of ovarian steroidogenic genes ( LHCGR, FSHR, StAR, CYP11A1 ), while modulated ovarian cholesterol metabolism via PPARα-mediated pathways. These findings elucidate the mechanisms by which GABA and L. plantarum 1-2-3 alleviate stress in laying hens, providing a theoretical and practical basis for their use as functional feed additives in stress-exposed flocks.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiamei Liu,Jian Zhao,Zhenyan Lu,Guichao Tian,Hong Zhang,Chao Jia,Meifang Zhu
The inherent hydrophilicity of cotton fabrics (CFs) limits their application in advanced functional textiles. Superhydrophobic surface modification has emerged as an effective strategy to impart functionalities such as self-cleaning and oil-water separation. Here, a two-step surface modification approach—combining graft condensation with free radical copolymerization—is developed to fabricate superhydrophobic CFs. The resulting fabrics exhibit a high water contact angle of 163° and a low sliding angle of 7°, indicating excellent surface hydrophobicity. The hydrophobic layer demonstrates exceptional mechanical robustness and chemical stability, maintaining a contact angle above 144° after 150 abrasion and 30 washing cycles. Furthermore, the hydrophobicity remains stable (contact angle >150°) under harsh conditions, including exposure to organic solvents, UV irradiation, elevated temperatures, and acidic/alkaline environments. The modified CFs also exhibit excellent self-cleaning performance and maintain oil-water separation efficiency exceeding 98 % after 20 cycles. These results highlight the potential of the developed modified CFs for applications in outdoor gear, anti-fouling textiles, and marine oil spill remediation.
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CHEMICAL PHYSICS LETTERS
·
2025
·
IF 3.1
Ciming Wang,Pengrui Zhang,Lianjing Mao,Chunsen Ye,Tianyu Zheng,Xiao Li,Wei Sun,Jinhe Sun
This study systematically investigates the competitive effects of H + , NH₄ + , and OH − on Li + migration and isotope separation during electromigration. Combined experimental and DFT analyses reveal that H + and NH₄ + significantly inhibit both Li + transport and isotopic partitioning, primarily through competitive chelation with 4-aminobenzo-15-crown-5, with H + exhibiting the strongest interference. DFT results confirm the binding affinity order: H + > NH₄ + > Li + . Additionally, OH − accumulation further impedes Li + migration and system stability. These findings highlight the critical importance of controlling electrolyte pH and minimizing competing ion concentrations, providing essential guidance for optimizing high-efficiency lithium isotope separation systems.
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Horticulturae
·
2025
·Vol. 11
·期 11
·1280
·
IF 3
Renchi Chen,Yuhua Yan,Weiliang Guan,Zhihai Huang
Both abscisic acid (ABA) and ethylene play essential roles in the ripening process of strawberry fruit (Fragaria×ananassaDuch.). In this study, the crosstalk between ABA and ethylene involved in strawberry fruit ripening was investigated. The results showed that ethylene triggered a 1.30-fold increase in ABA levels by suppressing the expression of miR396. Meanwhile, the silencing of miR396 (STTM396 reporter) demonstrated that miR396 acts as a negative regulatory factor of ABA biosynthesis by repressingFaNCED2expression. Additionally, dual-luciferase assays indicated a 0.56-fold suppression ofFaNCED2promoter activity by miR396. These findings reveal a novel regulatory mechanism in which ethylene promotes ABA biosynthesis through the suppression of miR396 expression in postharvest strawberry fruit.
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MOLECULES
·
2025
·Vol. 30
·期 21
·4157
·
IF 4.6
Yitong Sun,Lei Zhao,Ning Mi,Jiahao He,Jiantie Xu
Silicon (Si) is a promising high-capacity anode material for lithium–ion batteries but faces challenges such as severe volume fluctuations during cycles and the formation of unstable solid-electrolyte interphase films on the electrode surface. To address these limitations, we developed a bioinspired Si@C composite anode through polydopamine-mediated self-assembly of aromatic polyamide nanofibers and nano–Si, followed by controlled pyrolysis at 1000 °C under N2. The resulting hierarchical architecture mimics the symbiotic root-nodule structure of legumes, featuring vascular bundle-like carbon frameworks and chemically bonded Si/C interfaces. The optimized composite delivers an initial capacity of 1107.0 mAh g−1at 0.1 A g−1and retains 580.0 mAh g−1after 100 cycles with 52.4% retention. The exceptional electrochemical properties arise from the optimized architecture and surface interactions. The nature-inspired carbon network minimizes ionic transport resistance via vertically aligned porous pathways while simultaneously boosting lithium–ion adsorption capacity. Furthermore, radially aligned graphitic ribbons are generated through controlled polyamide thermal transformation that effectively mitigates electrode swelling and maintains stable interfacial layers during cycling.
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Polymers
·
2025
·Vol. 17
·期 20
·2762
·
IF 4.9
Minyue Shen,Lin Qi,Li Zhang,Panfei Ma,Wei Liu,To Ngai,Hang Jiang
Pickering emulsions have emerged as promising multiphase systems owing to their high stability and diverse applications in materials and chemical engineering. However, achieving precise and stimuli-responsive regulation of emulsion type, particularly reversible phase inversion between oil-in-water and water-in-oil states under fixed formulation without additional stabilizers, remains a considerable challenge. In this work, we developed a sol–gel strategy, i.e., in situ hydrolysis and condensation of silane precursors to form a silica shell directly on responsive microgels, to produce H-SiO2@P(NIPAM-co-MAA) hybrid microgels. The resulting hybrid particles simultaneously retained pH and temperature responsiveness, enabling the transfer of these properties from the polymeric network to the emulsion interface. When employed as stabilizers, the hybrid microgels allowed the controlled formation of Pickering emulsions that remained stable for one week under testing conditions. More importantly, they facilitated in situ reversible phase inversion under external stimuli. Overall, this work establishes a sol–gel approach to fabricate organic–inorganic hybrid microgels with well-defined dispersion and uniform silica deposition, while preserving dual responsiveness and enabling controlled phase inversion of Pickering emulsions.
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Foods
·
2025
·Vol. 14
·期 21
·3611
·
IF 5.1
Tingting You,Zhiguo Na,Ruobing Zhao,Yongqiang Ma
Lutein is one of carotenoids in the human brain that is consistently associated with all cognitive performance indicators, and its levels are closely linked to age-related cognitive decline. However, lutein application is limited by its poor stability and low bioaccessibility. In this study, a lutein-loaded delivery system was developed to enhance stability and achieve brain-targeting effects. Using high-speed shear and ethanol hydration methods, PEGylated lutein liposomes with lactoferrin (Lf-LLips) were constructed and characterized. The morphology was observed using TEM and AFM. Particle sizes and lutein retention rates were evaluated under different temperatures (4 °C, 25 ± 2 °C, 50 °C), light (diffusion light, DL; light shielding, LS), and storage durations at 28 d. Compared with free lutein, the in vitro release behavior and permeability across the blood–brain barrier of the systems were investigated. Lf-LLips exhibited a particle size of 186.63 ± 2.04 nm and a potential of −30.53 ± 1.65 mV, and the lutein encapsulation efficiency was 83.11 ± 1.67%. When stored under LS, the particle size of Lf-LLips remained under 190 nm at 4 °C for 28 days, and the retention rate of lutein exceeded 80%. The release curve of Lf-LLips in vitro over 72 h followed the Weibull model. Furthermore, the permeability across the blood–brain barrier model within 12 h was 22.73 ± 1.42%. These results demonstrate that Lf-LLips significantly improve the stability of lutein and exhibit sustained-release properties along with brain-targeting efficiency. The findings demonstrate the promising future of lutein for applications in brain health enhancement.
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Pathogens
·
2025
·Vol. 14
·期 10
·1069
·
IF 3.3
Xianqi Peng,Xialian Bu,Weisong Ma,Jinbiao Jiao,Xiaohong Huang,Yu Zhao,Jian Zhu,Lei Huang,Jing Chen,A’qin Zheng,Huantao Qu,Jiayun Yao
White spot disease, caused by the parasiticIchthyophthirius multifiliis, induces mortality exceeding 80% in intensive aquaculture systems, resulting in global annual losses of >$1 billion. Current chemical controls (e.g., formaldehyde) face environmental persistence and drug resistance challenges. This study developed a synthetic isoquinoline derivative, BHTCA (s)-2-tert-butoxycarbonyl-7-hydroxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid), as a biodegradable alternative againstI. multifiliisin grass carp (Ctenopharyngodon idella). In vitro assays revealed stage-selective efficacy: theronts exhibited higher susceptibility (EC50= 0.10 mg/L at 4 h) than tomonts (EC50= 0.40 mg/L at 24 h), with BHTCA disrupting ciliary structures and compromising cyst integrity. In vivo trials (0.6 mg/L) reduced parasite loads by 78.1% and host mortality by 66.7% versus untreated controls. Acute toxicity tests confirmed a high therapeutic index (LC50= 16.75 mg/L; >167× effective concentration). With demonstrated efficacy, low production cost, and minimal eco-toxicity, BHTCA presents a sustainable strategy for Ichthyophthiriasis management in aquaculture.
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