计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细菌的生化试验(也称生化反应)是指由于不同细菌具有各自的酶系统,对底物的分解能力不同,由此产生的代谢产物也不同,通过生物化学的方法测定这些代谢产物的过程,生化试验主要包括碳水化合物的生化试验、氨基酸和蛋白质的代谢试验、碳源和氮源的利用试验、酶类的代谢试验等,不同的细菌对蛋白质的分解能力不同,一般先由胞外酶把蛋白质分解为短肽或氨基酸,侵入细菌体内后由胞内酶把肽类分解为氨基酸。
甲基红试验试剂 (MR试剂) 有效成分为甲基红,由橘黄色变为红色则为阳性,适用于多种细菌的鉴定。该试剂仅用于科研领域,不宜用于临床诊断或其他用途。
自备材料:
1、试管或离心管、接种环、恒温培养箱
2、葡萄糖蛋白胨水培养基
操作步骤(仅供参考):
1、将待检细菌接种于 葡萄糖蛋白胨水培养基,置于恒温培养箱35~37°C培养24~ 48h。
2、取出培养液,沿管壁加入MR试剂1~2滴。
3、由橘黄色变为红色则为阳性,无色为阴性。
染色结果:
| 红色 | 阳性 |
| 颜色无变化 | 阴性 |
注意事项:
1、培养细菌时,注意避免交叉污染。
2、各试剂注意密闭保存,一旦开封,保质期就会大大缩短。
3、甲基红试验试剂仅用于科研目的,不可用于临床诊断。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用,以防影响后续实验效果。
Biochemical tests (also known as biochemical reactions) of bacteria refer to the process of determining metabolic products by biochemical methods, as different bacteria have their own enzyme systems, varying in the ability to decompose substrates and thus producing different metabolic products. Biochemical tests mainly include carbohydrate biochemical tests, amino acid and protein metabolism tests, carbon and nitrogen source utilization tests, and enzyme metabolism tests. Different bacteria differ in their ability to decompose proteins; generally, extracellular enzymes first decompose proteins into short peptides or amino acids, which then invade bacterial cells and are further decomposed into amino acids by intracellular enzymes.
Methyl Red Test Reagent (MR Reagent) has methyl red as the active ingredient; a color change from orange to red indicates a positive result, which is suitable for the identification of various bacteria. This reagent is for research use only and not suitable for clinical diagnosis or other purposes.
Materials to Be Prepared by the User:
1. Test tubes or centrifuge tubes, inoculation loops, constant temperature incubator
2. Glucose peptone water medium
Operating Procedures (For Reference Only):
1. Inoculate the test bacteria into the glucose peptone water medium, and incubate at 35–37℃ in a constant temperature incubator for 24–48 hours.
2. Take out the culture solution and add 1–2 drops of MR Reagent along the tube wall.
3. A color change from orange to red indicates a positive result; a colorless appearance indicates a negative result.
Staining Results:
| Red | Positive |
| No color change | Negative |
Precautions:
1. When culturing bacteria, take care to avoid cross-contamination.
2. All reagents should be stored in sealed containers. Once opened, their shelf life will be significantly shortened.
3. The Methyl Red Test Reagent is for research purposes only and not for clinical diagnosis.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
5. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | M1508186 |
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