计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细菌的生化试验(也称生化反应)是指由于不同细菌具有各自的酶系统,对底物的分解能力不同,由此产生的代谢产物也不同,通过生物化学的方法测定这些代谢产物的过程,生化试验主要包括碳水化合物的生化试验、氨基酸和蛋白质的代谢试验、碳源和氮源的利用试验、酶类的代谢试验等,不同的细菌对蛋白质的分解能力不同,一般先由胞外酶把蛋白质分解为短肽或氨基酸,侵入细菌体内后由胞内酶把肽类分解为氨基酸。
淀粉水解试验试剂基本原理是微生物如能产生淀粉酶(胞外酶),可将培养基中的淀粉水解为麦芽糖、葡萄糖等,再被细胞吸收利用,淀粉水解后遇碘不再变蓝,依此判断某些细菌有无分解淀粉的能力。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
1、淀粉培养基
2、接种环、恒温培养箱
操作步骤(仅供参考):
1、将待检细菌接种于淀粉培养基平板,置于恒温培养箱37°C培养2~3天。
2、打开培养皿,滴加淀粉水解试验试剂,轻轻摇动培养皿使碘液均匀铺满整个培养皿。
3、观察菌落周围是否出现无色透明圈以及透明圈大小,透明圈越大说明细菌对淀粉水解能力越大。
染色结果:
| 有无色透明圈 | 阳性 |
| 无变化 | 阴性 |
注意事项:
1、培养细菌时,注意避免交叉污染。
2、试剂注意密闭保存,一旦开封,保质期就会大大缩短。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
4、试剂开封后请尽快使用,以防影响后续实验效果。
Bacterial biochemical tests (also known as biochemical reactions) refer to the process of determining metabolic products through biochemical methods. This is based on the fact that different bacteria possess distinct enzyme systems, leading to varying abilities to decompose substrates and thus producing different metabolic products. Biochemical tests mainly include carbohydrate biochemical tests, amino acid and protein metabolism tests, carbon and nitrogen source utilization tests, enzyme metabolism tests, etc. Different bacteria exhibit different capacities for protein decomposition. Generally, extracellular enzymes first break down proteins into short peptides or amino acids, which then enter bacterial cells and are further decomposed into amino acids by intracellular enzymes.
The basic principle of the reagents for the starch hydrolysis test is as follows: if a microorganism can produce amylase (an extracellular enzyme), it can hydrolyze the starch in the culture medium into maltose, glucose, and other substances, which are then absorbed and utilized by the cells. After starch is hydrolyzed, it no longer turns blue when exposed to iodine. This principle is used to determine whether certain bacteria have the ability to decompose starch. This reagent is for research use only and is not suitable for clinical diagnosis or other applications.
Materials to Be Prepared by the User:
1. Starch culture medium
2. Inoculation loops, constant temperature incubator
Operating Procedures (For Reference Only):
1. Inoculate the test bacteria onto the starch culture medium plate and incubate it in a constant temperature incubator at 37°C for 2–3 days.
2. Open the Petri dish, add the reagent for the starch hydrolysis test, and gently shake the dish to ensure the iodine solution evenly covers the entire surface of the medium.
3. Observe whether a colorless transparent zone appears around the colonies and measure the size of the zone. A larger transparent zone indicates a stronger starch-hydrolyzing ability of the bacteria.
Staining Results:
| A colorless transparent zone is present | Positive |
| No change observed | Negative |
Precautions:
1. When culturing bacteria, care should be taken to avoid cross-contamination.
2. The reagent should be stored in a sealed container. Once opened, its shelf life will be significantly shortened.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
4. Please use the opened reagent as soon as possible to prevent it from affecting the results of subsequent experiments.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1508169 |
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