计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细菌的荚膜具有保护细菌抵抗吞噬和消化的作用,可增加细菌的侵袭力,荚膜不易被普通染色法染色,需经特殊染色法染色后才能着色,易于观察。细菌荚膜的鉴定,对细菌的分型和鉴别有至关重要的作用。
荚膜染色液(墨汁法)采用优质墨汁染色,用于观察菌体有无荚膜结构,借此鉴定某些细菌,如新型隐球菌,染色后新型隐球菌的荚膜取代了墨汁中的胶状碳粒,菌体被清楚无色透明的晕圈环所绕着。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
载玻片、盖玻片、酒精灯、显微镜
操作步骤(仅供参考):
1、将细菌与1滴染色液在载玻片上混合,加盖盖玻片。
2、轻轻压下, 使细菌混合液变薄。
3、在低倍镜下寻找有荚膜的细菌,并转高倍镜或油镜下仔细辨认。
染色结果:
| 新型隐球菌 | 宽厚透亮的荚膜,细胞壁明显 |
| 背景 | 黑色 |
注意事项:
1、有效期内已开封的染色液应在6个月内用完,每次用后尽量密封好,以免挥发或变质。
2、存储时间较长后易产生沉淀, 如果镜下发现颗粒粗糙者应过滤后使用或弃用。
3、目的样本尽量 新鲜。样本和荚膜染色液分布应均匀。
4、样本的纯度和浓度应适宜,浓度过低时在镜下很难寻找到新型隐球菌。
5、为了您的安全和健康,请穿实验服并戴-次性手套操作。
The capsule of bacteria can protect them against phagocytosis and digestion, and enhance their invasiveness. The capsule is not easily stained by ordinary staining methods and requires special staining techniques to be visualized for observation. The identification of bacterial capsules plays a crucial role in bacterial typing and differentiation.
Capsule Staining Solution (India Ink Method) uses high-quality India ink for staining. It is designed to observe the presence or absence of capsule structures on bacterial cells, thereby identifying certain bacteria, such as Cryptococcus neoformans. After staining, the capsule of Cryptococcus neoformans displaces the colloidal carbon particles in the India ink, and the bacterial cells are surrounded by distinct colorless and transparent halos. This reagent is for research use only (RUO) and is not suitable for clinical diagnosis or other applications.
Materials to Be Prepared by the User:
Glass slides, coverslips, alcohol lamps, microscopes
Operating Procedures (For Reference Only):
1. Mix the bacterial sample with 1 drop of the staining solution on a glass slide, then cover it with a coverslip.
2. Press gently to thin the bacterial mixture.
3. Search for capsulated bacteria under the low-power microscope, then switch to the high-power or oil immersion lens for detailed identification.
Staining Results:
| Cryptococcus neoformans | Thick, broad and transparent capsule with a distinct cell wall |
| Background | Black |
Precautions:
1. Opened staining solution within its expiration date should be used up within 6 months. Seal it tightly as much as possible after each use to prevent volatilization or deterioration.
2. Precipitation is likely to occur after long-term storage. If coarse particles are observed under the microscope, the solution should be filtered before use or discarded.
3. The target samples should be as fresh as possible. The samples and capsule staining solution must be evenly distributed.
4. The purity and concentration of the samples should be appropriate. It will be difficult to detect Cryptococcus neoformans under the microscope if the concentration is too low.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1508185 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for microbiology 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →