计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
地衣红又称苔红素,CAS号1400-62-0,分子量为500.51,是生物媒染剂,可以对HbsAg和鞭毛等进行染色。
地衣红乙醇溶液 (1%) 又称酸化地衣红染色液,主要由地衣红、盐酸、乙醇、去离子水等配制而成,溶液呈酸性,操作简单、特异性高,但染色效果可能受到不同批次原料而有差异。可用于乙肝病毒表面抗原(HBsAg)染色,也可以用于脂褐素和弹性纤维的染色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、组织固定于10%的中性福尔马林固定液,常规脱水包埋。
2、切片厚4µm,常规二甲苯或环保脱蜡透明液脱蜡至水。
3、入酸性高锰酸钾溶液(自备)氧化5min,稍水洗。
4、入1%草酸溶液(自备)漂白2min,脱去高锰酸钾着色,流水冲洗2min。
5、入70%乙醇稍浸洗。
6、入地衣红乙醇溶液加盖浸染5h或更长时间。
7、70%乙醇浸洗2次,每次10~30s,至切片无染液脱出为止。
8、常规脱水,二甲苯或环保脱蜡透明液透明,中性树胶封固。
注意事项:
1、染色效果可能受到不同批次原料而有差异。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Orcein, also known as lichenin, has a CAS number of 1400-62-0 and a molecular weight of 500.51. It is a biological mordant that can be used for staining HBsAg, flagella, and other structures.
Lichen Red Ethanol Solution (1%), also known as Acidified Orcein Staining Solution, is mainly prepared from orcein, hydrochloric acid, ethanol, deionized water and other components. The solution is acidic, featuring simple operation and high specificity, while the staining effect may vary with different batches of raw materials. It can be used for the staining of Hepatitis B Virus Surface Antigen (HBsAg), as well as the staining of lipofuscin and elastic fibers. This reagent is intended for research use only and not for clinical diagnosis or any other purposes.
Operating Procedures (For Reference Only):
1. Fix the tissues in 10% neutral formalin fixative, followed by routine dehydration and embedding.
2. Cut the sections at a thickness of 4 µm, then perform routine dewaxing to water with xylene or environment-friendly dewaxing and clearing solution.
3. Immerse the sections in acidified potassium permanganate solution (self-prepared) for oxidation for 5 minutes, then rinse briefly with water.
4. Transfer the sections into 1% oxalic acid solution (self-prepared) for bleaching for 2 minutes to remove the coloration caused by potassium permanganate, followed by rinsing under running water for 2 minutes.
5. Rinse the sections briefly in 70% ethanol.
6. Place the sections into orcein ethanol solution, cover them, and stain for 5 hours or longer.
7. Rinse the sections twice in 70% ethanol, 10–30 seconds each time, until no staining solution elutes from the sections.
8. Conduct routine dehydration, clear the sections with xylene or environment-friendly dewaxing and clearing solution, and mount the sections with neutral gum.
Precautions:
1. The staining effect may vary depending on different batches of raw materials.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
H302: 吞食有害
P501: 将内容物/容器处理到。。。
P264: 处理后要彻底洗手。
P270: 使用本产品时,请勿进食、饮水或吸烟。
P330: 漱口
P301+P317: 如果被吞咽:请寻求医疗帮助。
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1508167 |
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