计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品为非冻型血液DNA保存液,可有效保护血液中的DNA完整性。采集的血液样本可直接置于本保存液中,无需冻存即可实现DNA的稳定保存,常温条件下可达到接近冻存血液样本的DNA保存效果。本品极大便利了采样操作,可支持采样者在任意时间进行采样与样本保存,同时适用于血液样本的长途运输,广泛满足科研实验中的样本处理需求。
产品特点
操作简便:无需复杂步骤,将血液样品与本产品按指定比例混合即可完成保存准备。
适用范围广:可适配各种动物血液样本,兼容性强。
保存时间长:在4-25℃条件下,可稳定保存血液中的DNA长达六个月,尤其适合野外等无低温保存条件的场景下进行样本采集。
DNA完整性佳:保存后的血液样本可用于总DNA提取,提取所得DNA可直接应用于酶切、电泳、PCR等各类分子生物学实验。
使用方法
一、哺乳动物血液(含人血液)
取1-10 mL抗凝血,加入至适宜容量的塑料离心管中。
向离心管中加入等体积的血液DNA保存液,充分振荡,确保混合均匀。
将混合后的样本置于常温、闭光环境下保存,直至后续使用。
DNA提取步骤:取部分保存的混合液体,加入自备蛋白酶K(终浓度为0.1 mg/mL),37℃条件下处理过夜;随后采用经典的醇/氯仿抽提+乙醇沉淀方法,即可获得纯化DNA。
二、禽类血液Products
先用等体积的水将血液DNA保存液稀释。
将适量禽类血液直接加入稀释后的保存液中,充分振荡混合均匀。
后续保存及DNA提取操作,与“哺乳动物血液”使用方法一致。
注意事项
2. 保存液与血液样本需充分混合,否则可能导致局部DNA保存不充分。
3. 样本保存期间请保持闭光,避免光照对DNA造成降解。
This product is a non-freezing blood DNA preservation solution that effectively maintains the integrity of DNA in blood. Collected blood samples can be directly placed into this preservation solution, allowing for stable DNA preservation without the need for freezing. Under room temperature conditions, it achieves DNA preservation effects comparable to frozen blood samples. This product greatly facilitates sampling operations, enabling sample collection and preservation at any time, and is also suitable for long-distance transportation of blood samples. It widely meets the sample processing needs in scientific research experiments.
Product Features
Easy to Operate: No complex steps are required; simply mix the blood sample with this product in the specified ratio to complete the preservation process.
Wide Applicability: Compatible with various animal blood samples, offering strong adaptability.
Long Preservation Time: Under conditions of 4–25°C, DNA in blood can be stably preserved for up to six months, making it especially suitable for sample collection in scenarios such as fieldwork where low-temperature conditions are unavailable.
High DNA Integrity: Preserved blood samples can be used for total DNA extraction, and the extracted DNA can be directly applied to various molecular biology experiments, such as enzyme digestion, electrophoresis, and PCR.
Usage Instructions
For Mammalian Blood (Including Human Blood)
Take 1–10 mL of anticoagulated blood and add it to a plastic centrifuge tube of appropriate capacity.
Add an equal volume of Blood DNA Preservation Solution to the centrifuge tube and shake thoroughly to ensure even mixing.
Store the mixed sample at room temperature in a light-protected environment until further use.
DNA Extraction Procedure: Take an aliquot of the preserved mixture, add self-provided Proteinase K (final concentration of 0.1 mg/mL), and incubate overnight at 37°C. Subsequently, use the classic phenol/chloroform extraction and ethanol precipitation method to obtain purified DNA.
For Avian Blood
The number of nucleated cells in avian blood is significantly higher than in mammalian blood, so the volume of blood used should only be 1/100 to 1/1000 of that used for mammalian blood.
First, dilute the Blood DNA Preservation Solution with an equal volume of water.
Add an appropriate amount of avian blood directly to the diluted preservation solution and shake thoroughly to mix evenly.
Subsequent preservation and DNA extraction procedures are the same as those for mammalian blood.
Precautions
This reagent is intended for research use only and is strictly prohibited for clinical diagnosis or other non-research purposes.
The preservation solution and blood sample must be thoroughly mixed; otherwise, inadequate DNA preservation may occur in localized areas.
During sample preservation, ensure protection from light to prevent DNA degradation caused by light exposure.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N1518658 | |
| 分析证书 | N1518658 |
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