计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品特点
操作简便:无需复杂步骤,直接将新鲜组织放入本保存液中即可完成保存准备;无需使用液氮、干冰或-80℃冰箱,尤其适用于临床和野外样品的快速、大规模采集。
保存时间长:在4-25℃条件下,可稳定保存组织中的DNA长达六个月,无需低温环境即可实现长期保存。
DNA完整性佳:保存后的组织样本可用于总DNA提取,提取所得DNA可直接应用于酶切、电泳、PCR等各类分子生物学实验,保障实验结果的可靠性。
使用方法
用量配比:100 mg组织约需1 mL组织DNA保存液,请根据所处理组织的重量,估算完全浸没样品所需的保存液体积。
样品处理:在做好标记的收集管中,加入估算量的本保存液,将组织碎块放入保存液中,确保组织碎块完全浸没于保存液内,避免部分组织暴露。
样本保存:将装有样品和保存液的收集管,存放于适宜温度环境,保存温度不得低于4℃。
后续操作:DNA提取时,按常规的DNA提取流程进行即可,无需额外处理保存后的组织样本。
注意事项
2. 若1mL保存液内同时保存多块组织标本,虽可起到一定保护作用,但组织细胞内会残留水分,会缩短保存期限;若需长期保存,请尽快将每块组织分装至新的保存液中,或在原管内按比例增加保存液用量,确保组织完全浸没。
3. 组织样本需新鲜采集后立即放入保存液中,避免组织变质影响DNA完整性;保存期间请保持收集管密封,防止保存液挥发。
This product is a Non-freezing tissue DNA preservation solution, suitable for preserving DNA in fresh tissue under room temperature conditions. It greatly facilitates sample collection and preservation in scenarios such as field studies and operating rooms, as well as the transportation of samples at room temperature. This non-denaturing solution effectively maintains the integrity of DNA molecules by inhibiting nuclease activity without altering the original structure of biomacromolecules. It widely meets the needs of tissue sample processing and preservation in scientific research experiments.
Product Features
Easy to Operate: No complex steps are required; simply place fresh tissue directly into the preservation solution to complete the preservation process. No need for liquid nitrogen, dry ice, or a -80°C freezer, making it especially suitable for rapid, large-scale collection of clinical and field samples.
Long Preservation Time: Under conditions of 4–25°C, DNA in tissue can be stably preserved for up to six months, enabling long-term preservation without the need for low-temperature environments.
High DNA Integrity: Preserved tissue samples can be used for total DNA extraction, and the extracted DNA can be directly applied to various molecular biology experiments, such as enzyme digestion, electrophoresis, and PCR, ensuring reliable experimental results.
Usage Instructions
Dosage Ratio: Approximately 100 mg of tissue requires about 1 mL of Tissue DNA Preservation Solution. Estimate the volume of preservation solution needed to completely submerge the sample based on the weight of the tissue being processed.
Sample Processing: Add the estimated amount of preservation solution to a labeled collection tube. Place the tissue fragments into the preservation solution, ensuring that the tissue fragments are completely submerged and no part of the tissue is exposed.
Sample Preservation: Store the collection tube containing the sample and preservation solution in an appropriate temperature environment, ensuring the storage temperature does not fall below 4°C.
Subsequent Operations: For DNA extraction, follow the routine DNA extraction procedure without any additional processing of the preserved tissue samples.
Precautions
This reagent is intended for research use only and is strictly prohibited for clinical diagnosis or other non-research purposes.
If multiple tissue specimens are stored together in 1 mL of preservation solution, some protective effect may still be provided. However, residual water within the tissue cells may shorten the preservation period. For long-term storage, promptly transfer each tissue piece into fresh preservation solution or proportionally increase the volume of preservation solution in the original tube to ensure the tissue is completely submerged.
Tissue samples should be freshly collected and immediately placed into the preservation solution to prevent tissue degradation from affecting DNA integrity. During storage, keep the collection tube sealed to prevent volatilization of the preservation solution.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N1520267 | |
| 分析证书 | N1520267 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 → 查看 Suitable for molecular biology 级别指南 →