计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品特点
操作简便:无需复杂步骤,直接将新鲜细菌放入本保存液中即可完成保存准备;无需使用液氮、干冰或-80℃冰箱,尤其适用于临床和野外样品的快速、大规模采集。
保存时间长:在4-25℃条件下,可稳定保存细菌中的RNA长达15天,无需低温环境即可实现短期高效保存。
RNA完整性佳:保存后的细菌样本可用于总RNA提取,提取所得RNA可直接应用于反转录、一步法RT-PCR等各类分子生物学实验,保障实验结果的可靠性。
使用方法
菌液处理:取1 mL过夜培养的菌液,置于室温条件下,以10,000 rpm转速离心1分钟,离心后弃去上清液。
样本保存:向离心后的菌体中加入1 mL非冻型细菌RNA保存液,充分混匀,确保菌体在保存液中完全、均匀分散,无团聚现象。
样本转移:将上述混匀后的溶液,转移至做好标记的收集管中,盖紧管盖。
保存条件:将装有样品和保存液的收集管存放于适宜温度环境,若需长期保存,保存温度不得低于4℃。
后续操作:RNA提取时,按常规的细菌RNA提取流程进行即可,无需额外处理保存后的细菌样本。
注意事项
2. 操作过程中请尽量在无菌条件下进行,避免外源RNase(核糖核酸酶)污染;虽然保存液本身可抑制RNase活性,但引入其他微生物会增加RNA降解的风险,同时增加后续实验分析的复杂性。3. 若初始菌液浓度过高,或需长期保存(如数月以上),请按比例增加保存液用量,或减少用于保存的菌体量,确保菌体得到充分保护,避免RNA降解。
This product is a non-freezing bacterial RNA preservation solution, suitable for preserving RNA in bacteria under room temperature conditions. It greatly facilitates sample collection and preservation in scenarios such as field studies and operating rooms, as well as the transportation of samples at room temperature. This non-denaturing solution effectively maintains the integrity of RNA molecules by inhibiting nuclease activity without altering the original structure of biomacromolecules. It widely meets the needs of bacterial sample processing and preservation in scientific research experiments.
Product Features
Easy to Operate: No complex steps are required; simply place fresh bacteria directly into the preservation solution to complete the preservation process. No need for liquid nitrogen, dry ice, or a -80°C freezer, making it especially suitable for rapid, large-scale collection of clinical and field samples.
Long Preservation Time: Under conditions of 4–25°C, RNA in bacteria can be stably preserved for up to 15 days, enabling efficient short-term preservation without the need for low-temperature environments.
High RNA Integrity: Preserved bacterial samples can be used for total RNA extraction, and the extracted RNA can be directly applied to various molecular biology experiments, such as reverse transcription and one-step RT-PCR, ensuring reliable experimental results.
Usage Instructions
Bacterial Culture Processing: Take 1 mL of an overnight bacterial culture and place it at room temperature. Centrifuge at 10,000 rpm for 1 minute, then discard the supernatant.
Sample Preservation: Add 1 mL of Non-Freezing Bacterial RNA Preservation Solution to the pelleted bacteria after centrifugation. Mix thoroughly to ensure the bacteria are completely and evenly dispersed in the preservation solution without any clumping.
Sample Transfer: Transfer the mixed solution to a labeled collection tube and tightly close the cap.
Storage Conditions: Store the collection tube containing the sample and preservation solution in an appropriate temperature environment. For long-term storage, ensure the storage temperature does not fall below 4°C.
Subsequent Operations: For RNA extraction, follow the routine bacterial RNA extraction procedure without any additional processing of the preserved bacterial samples.
Precautions
This reagent is intended for research use only and is strictly prohibited for clinical diagnosis or other non-research purposes.
During operation, please try to work under sterile conditions to avoid contamination by exogenous RNase (ribonuclease). Although the preservation solution itself can inhibit RNase activity, introducing other microorganisms may increase the risk of RNA degradation and add complexity to subsequent experimental analysis.
If the initial bacterial culture concentration is too high, or if long-term storage (e.g., several months or more) is required, proportionally increase the volume of preservation solution or reduce the amount of bacterial pellet used for storage to ensure the bacteria are fully protected and to avoid RNA degradation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | B1520266 | |
| 分析证书 | B1520266 |
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