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EnzymoPure™ 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 28 篇同行评审文献引用。
Description
Neutral protease from Clostridium histolyticum is a proteolytic enzyme and therefore can be used for isolation of various cell types by tissue dissociation.
Applications
Neutral protease suitable for cell isolation from various tissue types for clinical applications.
Specifications
Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.
Neutral protease activity (DMC): ≥ 0.50 U/mg
Endotoxin: ≤ 100 EU**/mg (Ph. Eur.)
Largely free of collagenolytic activity.
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).
Manufactured according to EU GMP guidelines.
Mr ca. 34 000 • CAS [9001-92-7]
References and Definitions
Unit definition:
*Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
**Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
References
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
Description
Neutral protease from Clostridium histolyticum is a proteolytic enzyme and therefore can be used for isolation of various cell types by tissue dissociation.
Applications
Neutral protease suitable for cell isolation from various tissue types for clinical applications.
Specifications
Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.
Neutral protease activity (DMC): ≥ 0.50 U/mg
Endotoxin: ≤ 100 EU**/mg (Ph. Eur.)
Largely free of collagenolytic activity.
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).
Manufactured according to EU GMP guidelines.
Mr ca. 34 000 • CAS [9001-92-7]
References and Definitions
Unit definition:
*Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
**Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
References
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.