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EnzymoPure™ 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 53 篇同行评审文献引用。
Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.Alkaline phosphatase is used for conjμgation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.
Optimum pH: 8.0 (Garen and Levinthal 1960).
Extinction coefficient: E278 = 7.2 (Anderson et al. 1975).
Isoelectric point: pH 4.5 (Garen and Levinthal 1960).
Activity: Bloch and Schlesinger (1974) report on kinetic studies. Bock and Sheard (1975) indicate that the E. coli enzyme binds phosphate tightly over a wide range of pH forming complexes that may be intermediate in the hydrolytic action. See also Bosron and Vallee (1975), Zukin and Hollis (1975), and Chlebowski and Coleman (1974).
Specificity: See review by Reid and Wilson (1971). Also Mushak and Coleman (1972) and Snyder and Wilson (1972).
Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates.
Stability: Stable at 2 - 8°C for at least 6 - 12 months.
Chromatographically purified. A suspension in 2.6M ammonium sulfate, pH 8.0.