计算溶液所需的质量、体积或浓度。
概述
磷酸盐缓冲液（PBS）是一种用于生物学研究的缓冲溶液。它是一种含有磷酸钠、氯化钠并在某些配方中含有氯化钾和磷酸钾的水基盐溶液。溶液的重量摩尔渗透压浓度和离子浓度与人体相同（等渗），并且对绝大多数细胞无毒。
产品应用：
磷酸缓冲盐已用于生产铂纳米颗粒(NP-Pt)水解胶体在SEM之前清洗血液样品和SEM（扫描电子显微镜）存根制备荧光标记小麦胚芽凝集素(WGA)溶液在ELISA（酶联免疫吸附测定）期间稀释捕获抗体在离心过程中悬浮细胞沉淀免疫测定程序
使用方法：
1.使用三级水或一级水溶解本品并定容至10ml，可得10ml体积的10X磷酸盐缓冲盐溶液浓缩液。2.若需配置1X浓度磷酸盐缓冲盐溶液，可用三级水或一级水溶解本品并定容至100ml，或将10X浓缩液与三级水或一级水按1:9比例进行稀释。3.溶解后所得磷酸盐缓冲盐溶液经0.22μm过滤除菌后，可用于细胞生物学实验，配制用水内毒素需低于0.01EU/ml并用去内毒素的无菌容器盛装。4.蒸气灭菌会导致溶液体积损失并造成溶液浓度,pH,渗透压等参数变化，请勿采用蒸气灭菌方法对溶液进行除菌。5.本品溶解后pH在25℃-37℃可稳定于所标注的范围内，无需使用HCl进一步进行调节，如需其他pH的磷酸盐缓冲盐溶液，可购买对应产品或进行定制。
产品规格与参数:
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Product introduction:
Phosphate buffer solution (PBS) is a kind of buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulas, potassium chloride and potassium phosphate. The weight molar osmotic pressure concentration and ion concentration of the solution are the same as those of human body (isotonic), and they are non-toxic to most cells.
Product application:
Phosphoric acid buffer salt has been used to produce platinum nanoparticles (NP Pt) hydrolytic colloid to wash blood samples before SEM and to prepare SEM (scanning electron microscope) stub to prepare fluorescent labeled wheat germ agglutinin (WGA) solution. During ELISA (enzyme-linked immunosorbent assay), dilute and capture antibodies. During centrifugation, suspend cells to precipitate immunoassay procedures
usage method:
1. Dissolve the product in Grade III water or Grade I water and dilute it to 10ml to obtain 10ml of concentrated 10X phosphate buffer salt solution. 2. If it is necessary to prepare 1X concentration phosphate buffer salt solution, the product can be dissolved in tertiary water or primary water to a constant volume of 100ml, or 10X concentrated solution can be diluted with tertiary water or primary water in a ratio of 1:9. 3. The phosphate buffer salt solution obtained after dissolution passes 0.22 μ M After filtration and sterilization, it can be used for cell biology experiment. The endotoxin in the preparation water should be less than 0.01EU/ml and be packed in a sterile container without endotoxin. 4. Steam sterilization will cause solution volume loss and change of solution concentration, pH, osmotic pressure and other parameters. Do not use steam sterilization to sterilize the solution. 5. After dissolution, the pH of the product can be stabilized within the marked range at 25 ℃ - 37 ℃, without further adjustment with HCl. If you need phosphate buffer salt solution of other pH, you can purchase the corresponding product or customize it.
Product specifications and parameters:
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规格
英文名称
Phosphate Buffered Saline Tablets
储存条件
室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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