肝靶向性活体高效转染试剂是一类针对体内靶向递送的转染试剂，根据客户需求能够将mRNA、siRNA靶向递送至肺部、脾、肝脏、脑部、肿瘤，实现mRNA的高效表达以及目的基因的siRNA沉默。
产品组分及储存条件：
P751603
| Components
| 100μL
| 500μL
| 1.5mL
| Storage
|
P751603A
| 肝靶向性活体高效转染试剂
| 100μL
| 500μL
| 1.5mL
| 2-8℃
|
P751603B
| Transfection Buffer
| 0.3mL
| 1.5mL
| 5mL
| 2-8℃ |
实验材料：
• 本产品，包含肝靶向性活体高效转染试剂和Transfection Buffer两种试剂
• ⾼纯度的核酸储液（0.5∼5 μg/μL）
质量控制：
• 4 °C保存条件下有效期一年
• 本产品经严格的质量检验证明，⽆⽣物污染
注意事项：
•常温下制备RNA-肝靶向性活体高效转染试剂复合物，使用灭酶灭菌管，孵育时间20～30min。
•本产品应该在4°C密闭保存，避免反复冻融。
•体内转染时，对于瘤内递送，可通过改变肿瘤体积的大小、mRNA的用量和浓度以及肝靶向性活体高效转染试剂用量对体内转染进行优化。
•转染试剂使用后请立即盖好盖子，避免长时间暴露在空气中，影响转染效率。
体内靶向递送示意图：
体内分别转染Firefly Luciferase mRNA和siRNA效果示例：
体内转染时，根据小鼠体重优化转染条件。肝靶向性活体高效转染试剂(μL)：RNA(μg)可在3：1到6：1间调整。对于瘤内递送，保证肿瘤体积在50mm³~200mm³ 最佳。
表1.不同体重鼠的转染用量
鼠体重
| 建议瘤体积
| 肝靶向性活体高效转染试剂
| RNA用量
|
15 g
| 100 mm³
| 49 μL
| 12 μg
|
20 g
| 100 mm³
| 65 μL
| 15 μg
|
25 g
| 100 mm³
| 82 μL
| 19 μg
|
30 g
| 100 mm³
| 98 μL
| 23 μg |
Liver-Targeted High-Efficiency In Vivo Transfection Reagent is a category of transfection reagents designed for in vivo targeted delivery. According to customer needs, it can target and deliver mRNA and siRNA to the lungs, spleen, liver, brain, and tumors, enabling efficient mRNA expression and siRNA silencing of the target gene.
Product Components and Storage Conditions:
P751603
| Components
| 100μL
| 500μL
| 1.5mL
| Storage
|
P751603A
| Liver-Targeted High-Efficiency In Vivo Transfection Reagent
| 100μL
| 500μL
| 1.5mL
| 2-8℃
|
P751603B
| Transfection Buffer
| 0.3mL
| 1.5mL
| 5mL
| 2-8℃ |
Experimental Materials:
• This product contains two reagents: Liver-Targeted High-Efficiency In Vivo Transfection Reagent and Transfection Buffer.
• High-purity nucleic acid stock solution (0.5–5 μg/μL)
Quality Control:
• The shelf life is 1 year when stored at 4 °C.
• Strict quality inspection has confirmed that this product is free from biological contamination.
Precautions:
• Prepare the RNA-Liver-Targeted High-Efficiency In Vivo Transfection Reagent complex at room temperature using enzyme-free and sterile tubes, with an incubation time of 20–30 minutes.
• This product should be stored tightly sealed at 4°C, and repeated freezing and thawing should be avoided.
• For in vivo transfection, in the case of intratumoral delivery, the in vivo transfection can be optimized by adjusting the tumor volume, the dosage and concentration of mRNA, and the dosage of Liver-Targeted High-Efficiency In Vivo Transfection Reagent.
• After using the transfection reagent, please close the lid immediately to avoid prolonged exposure to air, which may affect the transfection efficiency.
Schematic Diagram of In Vivo Targeted Delivery:
Example of effects of in vivo transfection with Firefly Luciferase mRNA and siRNA respectively:
For in vivo transfection, optimize the transfection conditions according to the weight of mice. The ratio of Liver-Targeted High-Efficiency In Vivo Transfection Reagent (μL) to RNA (μg) can be adjusted within the range of 3:1 to 6:1. For intratumoral delivery, it is optimal to keep the tumor volume within 50 mm³ - 200 mm³.
Table 1. Transfection Dosages for Mice of Different Body Weights
Mouse Weight
| Recommended Tumor Volume
| Dosage of Liver-Targeted High-Efficiency In Vivo Transfection Reagent
| Dosage of RNA
|
15 g
| 100 mm³
| 49 μL
| 12 μg
|
20 g
| 100 mm³
| 65 μL
| 15 μg
|
25 g
| 100 mm³
| 82 μL
| 19 μg
|
30 g
| 100 mm³
| 98 μL
| 23 μg |
ZJ26F0131238
危险品化学品经营许可证（带存储）