常见细胞转染试剂是一款通用型核酸转染试剂，能够将DNA、mRNA、siRNA等多种核酸高效转染至贴壁细胞、悬浮细胞，具有效率高、毒性低等优点，对HeLa、A549、DC 2.4等贴壁细胞以及293、CHO等悬浮细胞实现高转染效率。产品以无菌的液体形式提供。
通常情况下，使用推荐用量，每毫升本试剂大约可以转染6孔板200个孔、24孔板1000个孔、96孔板5000个孔。
实验材料：
• 常见细胞转染试剂
• ⾼纯度的核酸储液（0.5∼5 μg/μL）
• ⽆⾎清培养基（如 O778393 Optimal-MEM减血清培养基 (含酚红) ）
质量控制：
• 2-8℃保存条件下有效期⼀年，避免反复冻融
• 本产品经严格的质量检验证明，⽆⽣物污染
注意事项：
• 常温下制备常见细胞转染试剂-核酸复合物，孵育时间20~30min。转染时使用无血清培养基（如O778393 Optimal-MEM减血清培养基 (含酚红))和灭酶管。
• 利用本产品转染4-6h后，去除原培养基，更换新鲜含血清培养基。
• 初次使用需要优化核酸浓度与常见细胞转染试剂的比例以得到最高的转染效率。建议基于推荐的产品用量做进一步优化。
• 转染时请使用适当保存和经常传代的健康细胞，并确保培养基无细菌、真菌或支原体污染。如细胞是近期复苏的液氮冻存细胞，请在转染前至少传代两次。
• 转染试剂使用后请立即盖好盖子，避免长时间暴露在空气中，影响转染效率。
GFP-HeLa细胞的siRNA沉默效果示例：
图1. 常见细胞转染试剂和竞品在GFP-HeLa细胞中递送siRNA的基因沉默效果荧光显微镜图。
HeLa细胞转染效果示例：
图2. 常见细胞转染试剂和竞品在HeLa细胞中递送质粒和mRNA的转染效果荧光显微镜图和平均荧光强度柱状图。
悬浮细胞转染效果示例：
按照下表转染细胞。使用指定量的核酸以及对应的常见细胞转染试剂。每种反应混合物体积为单个孔的体积，且考虑了移液差异。按比例计算其他孔的体积。
The common cell transfection reagent is a universal nucleic acid transfection reagent. It can efficiently transfect various nucleic acids such as DNA, mRNA, and siRNA into adherent cells and suspension cells, and has the advantages of high efficiency and low toxicity. It achieves high transfection efficiency for adherent cells such as HeLa, A549, and DC 2.4, as well as suspension cells such as 293 and CHO. The product is supplied as a sterile liquid.
Under normal circumstances, when used at the recommended dosage, each milliliter of this reagent can approximately transfect 200 wells of a 6-well plate, 1000 wells of a 24-well plate, and 5000 wells of a 96-well plate.
Experimental Materials:
• Common cell transfection reagent
• High-purity nucleic acid stock solution (0.5∼5 μg/μL)
• Serum-free medium (e.g., O778393 Optimal-MEM Reduced Serum Medium (with Phenol Red) ).
Quality Control:
• The shelf life is one year under storage conditions of 2-8℃, Avoid freeze/thaw cycle.
• This product has passed strict quality inspection, proving that it is free from biological contamination.
Precautions:
• Prepare the common cell transfection reagent-nucleic acid complex at room temperature, with an incubation time of 20–30 minutes. For transfection, use serum-free medium (e.g., O778393 Optimal-MEM Reduced Serum Medium (with phenol red)) and enzyme-free tubes.
• After transfecting with this product for 4–6 hours, remove the original medium and replace it with fresh serum-containing medium.
• For first-time use, it is necessary to optimize the ratio of nucleic acid concentration to the common cell transfection reagent to achieve the highest transfection efficiency. It is recommended to conduct further optimization based on the recommended product dosage.
• During transfection, use healthy cells that are properly preserved and regularly passaged, and ensure that the medium is free from bacterial, fungal, or mycoplasma contamination. If the cells are recently revived from liquid nitrogen-frozen stocks, passage them at least twice before transfection.
• After using the transfection reagent, immediately close the cap tightly to avoid prolonged exposure to air, which may affect transfection efficiency.
Example of siRNA Silencing Effect in GFP-HeLa Cells:
Figure 1. Fluorescence microscopy images showing the gene silencing effect of siRNA delivered by the Common Cell Transfection Reagent and competing products in GFP-HeLa cells.
Example of Transfection Effect in HeLa Cells:
Figure 2. Fluorescence microscopy images and bar graphs of mean fluorescence intensity showing the transfection effect of plasmids and mRNA delivered by the Common Cell Transfection Reagent and competing products in HeLa cells.
Example of Transfection Effect in Suspension Cells:
Transfect the cells according to the table below. Use the specified amount of nucleic acid and the corresponding common cell transfection reagent. The volume of each reaction mixture is the volume for a single well, with pipetting differences taken into account. Calculate the volumes for other wells in proportion.
ZJ26F1039822
危险品化学品经营许可证（带存储）