重组葡萄糖氧化酶 (GOD)

Name: 重组葡萄糖氧化酶 (GOD)
SKU: R1505821
Availability: InStock

CAS: 9001-37-0 货号: R1505821 EC号: 232-601-0 PubChem CID: 64689

586篇引用文献 COA

有货

级别和纯度: 生物活性 ? 重组 ? ActiBioPure™ ? 高性能 ? EnzymoPure™ ? ≥180 U/mg enzyme powder

Accession #

P13006

表达系统

酵母

生物活性

≥180 U/mg enzyme powder

规格

库存

价格

数量

50KU

R1505821-50KU

现货

¥429.90

250KU

R1505821-250KU

现货

¥1,499.90

1MU

R1505821-1MU

现货

¥3,599.90

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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 586 篇同行评审文献引用。

概述

Glucose oxidase (GOD, EC 1.1.3.4) exhibits high specificity and can specifically catalyze the oxidation reaction of β-D-glucose. Its systematic name is β-D-glucose:oxygen 1-oxidoreductase. In the absence of catalase, the core of the reaction is that "β-D-glucose is oxidized by O₂ to gluconolactone, with the simultaneous production of H₂O₂".

Application

In clinical analysis, this enzyme can be used for the enzymatic determination of glucose; when maltosaccharides or modified starch are used as substrates, it can also be applied to the detection of amylase activity by coupling with α-glucosidase.

Assay procedure

Principle

The appearance of quinoneimine dye is measured at 555nm by spectrophotometry.

Unit definition

One unit causes the formation of one micromole of hydrogen peroxide(half a micromole of quinoneimine dye)per minute under the conditions described below.

Reagents

A. MES-Na buffer pH5.7:0.1mM [Dissolve 2.13g of 2-(N-morpholino)ethansulfonic acid (MW=213.25) in ca.60ml of H₂O and, after adjusting the pH5.7 with 1N NaOH at 25℃, fill up to 100ml with H₂O] (Stable at 5℃ for one month)

B. Glucose solution: 15% [Dissolve 1.5g of β-D-Glucose and fill up to 10ml with H₂O] for at least 2hrs before assay. (Should prepare fresh)

C. 4-AA solution: 0.5% [50mg of 4-aminoantipyrine (MW=203.25)/10ml of H₂O] (Stable at 5℃ in a brownish bottle for at least one week)

D. TOOS solution: 40mM [132.5mg of N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine (MW=331.36/10ml of H₂O] (Stable at 5℃ in a brownish bottle for at least one week)

E. Peroxidase solution: 500U (purpurogalin unit) /ml of H₂O

F. Enzyme diluent: 10mM MES-Na buffer, pH5.7, containing 0.1% Triton X-100

Procedure

Prepare the following working solution in a brownish bottle and store on ice. (Should be prepared fresh)

30ml	Buffer solution	A
6ml	Substrate solution	B
0.3ml	4-AA solution	C
0.3ml	TOOS solution	D
0.3ml	POD solution	E

Concentration in assay mixture

MES buffer	79mM
D-Glucose	131mM
4-AA	0.2mM
TOOS	0.3mM
POD	ca.4 U /ml

2. Pipette 3.0ml of working solution into a cuvette (d=1.0 cm) and equilibrate at 37℃ for about 5 minutes.

3. Add 0.1ml of the enzyme solution* and mix by gentle inversion.

4. Record the increase in optical density at 555nm against water for 2 to 3 minutes in a spectrophotometer thermostated at 37℃,and calculate the ∆OD per minute from the initial portion of the curve( △OD test).At the same time,measure the blank rate(∆OD blank)by using the same method as the test except that enzyme diluent (F) is added instead of the enzyme solution.

* Dissolve the enzyme preparation in ice-cold enzyme diluent(F)and dilute to 0.05~0.2U/ml with the same buffer,immediately before assay.

Calcuation

Activity can be calculated by using the following formula:

Vt:	Total volume (3.1ml)
Vs:	Sample volume (0.1ml)
32.8:	Millimolar extinction coefficient of quinoneimine dye under the assay conditions (cm²/μmol)
1.0:	Light path length (cm)
1/2:	Factor based on the fact that one mole of H₂O₂ produces a half of quinoneimine dye.
df:	Dilution factor
C:	Enzyme concentration in dissolution (C mg/ml)

规格

产品名称

重组葡萄糖氧化酶 (GOD)

英文别名

规格或纯度

生物活性, 重组, ActiBioPure™, 高性能, EnzymoPure™, ≥180 U/mg enzyme powder

生化机理

Glucose oxidase catalyzes the oxidation of beta-D-glucose to D-glucono-delta-lactone and hydrogen peroxide in the presence of molecular oxygen. D-glucono-delta-lactone is sequentially hydrolyzed by lactonase to D-gluconic acid, and the resulting hydrogen peroxide is hydrolyzed by catalase to oxygen and water. The activity shows high specificity to beta-D-glucose, with very low to no activity towards L-glucose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, 4-deoxy-D-glucose, 5-deoxy-D-glucose, 6-deoxy-D-glucose, 3-O-methyl-D-glucose, 4-O-methyl-D-glucose, 6-O-methyl-D-glucose, 4,6-O-benzylidene-D-glucose, 5-thio-5-deoxy-D-glucose, D-mannose, D-allose, D-galactose, D-fructose, D-arabinose, D-xylose, trehalose, melibiose, L-mannomethylose, lactose, sucrose or 1,5-anhydro-D-glucitol. Glucose oxidase was discovered by 26 years old Danish botanist Detlev Mueller in 1925. Detlev Mueller, at the then Royal Danish Veterinary and Agricultural University (KVL), published a brief note in a somewhat inaccessible journal reporting his discovery of a new enzyme, glucose oxidase (A new enzyme, glucose oxidase, from Aspergillus niger. Kgl. Veterinaer. og Landbohojskole Aarsskrift (Copenhagen) 1925, 329-331). Aspergillus niger glucose oxidase was the first flavoenzyme to be discovered, however, it was Nobel Prize winner Otto Warburg and Walter Christian, who in 1932-1933 discovered a different enzyme, glucose 6-phosphate dehydrogenase, in baker's yeast Saccharomyces cerevisiae, that did recognize the significance of their enzyme's yellow cofactor. Post-translational: The N-linked sugar chains of the glucose oxidase contributed to the high solubility of the enzyme in water.

生物活性

≥180 U/mg enzyme powder

表达系统

酵母

来源

重组表达

CAS编号和信息

9001-37-0

酶学委员会编号

EC 1.1.3.4

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥180 U/mg enzyme powder

复溶

Reconstitute in 10mM MES-Na buffer, pH5.7, containing 0.1% Triton X-100.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（24个月）；复溶后建议分装，避免反复冻融。

单位定义

1个酶活单位等于在37°C条件下1分钟生成1微摩尔过氧化氢（1/2毫摩尔醌染料）的酶量。

技术文档

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高级数据

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C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ26F0130802	分析证书	R1505821
ZJ26F0130800	分析证书	R1505821
ZJ26F0130801	分析证书	R1505821

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