Recombinant Human Activin A Protein

Name: Recombinant Human Activin A Protein
SKU: rp142493
Availability: InStock

货号: rp142493

COA

有货

级别和纯度: 无动物源 ? 无载体 ? 生物活性 ? ActiBioPure™ ? 无叠氮钠 ? 高性能 ? His标签 ? ≥95%(SDS-PAGE)

Accession #

P08476

蛋白标签

C-His

表达系统

HEK293

内毒素水平

<0.1 EU/μg

生物活性

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 2.87 ng/mL.

规格

库存

价格

数量

10μg

rp142493-10μg

现货

¥999.90

50μg

rp142493-50μg

现货

¥2,439.90

100μg

rp142493-100μg

现货

¥3,899.90

1mg

rp142493-1mg

期货

¥21,999.90

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概述规格储存与运输图片技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器常见问题

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为什么选择此级别

ActiBioPure™,His标签,无动物源,无叠氮钠,无载体,生物活性,高性能级提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Purity
≥95% (SDS-PAGE)
Endotoxin level
＜0.1 EU/μg
Function
Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins. Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively. The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4. Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription. The bioactivity of Activin A is regulated by a variety of mechanisms. BAMBI, Betaglycan, and Cripto are cell‑associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly. The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A. And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG. Activin A is involved in the differentiation of various cell and tissue types. The induction of definitive endoderm by Activin A is required in differentiation protocols of induced pluripotent stem cells (iPSCs). In vitro models of human gametogenesis use prolonged Activin A supplementation to human embryonic stem cells for differentiation into human primordial germ cell-like cells. Activin A can also be used to maintain cells in vitro, as is the case for iPSC-derived nephron cells that can then be used in disease modeling, drug screening and in regenerative medicine. Activin A is an important factor for tumor cells to evade the immune system as Activin A can act on surrounding immune cells to decrease their antitumor activity. Activin A also promotes migration and growth of tumors, making it a target for cancer therapies. Specifically, research has shown that interfering with Activin A activity can assist in overcoming CD8 T-cell exclusion and immunotherapy resistance. In bone marrow-derived stem cell transplants for treatment of diabetes, Activin A enhances migration and homing of stem cells towards pancreatic lineage.

规格

产品名称

Recombinant Human Activin A Protein

别名

重组激活素A蛋白

英文别名

规格或纯度

无动物源,无载体,生物活性,ActiBioPure™,无叠氮钠,高性能,His标签,≥95%(SDS-PAGE)

生物活性

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 2.87 ng/mL.

表达系统

HEK293

种属

人(Human)

氨基酸

21-426 aa

序列

SPTPGSEGHSAAPDCPSCALAALPKDVPNSQPEMVEAVKKHILNMLHLKKRPDVTQPVPKAALLNAIRKLHVGKVGENGYVEIEDDIGRRAEMNELMEQTSEIITFAESGTARKTLHFEISKEGSDLSVVERAEVWLFLKVPKANRTRTKVTIRLFQQQKHPQGSLDTGEEAEEVGLKGERSELLLSEKVVDARKSTWHVFPVSSSIQRLLDQGKSSLDVRIACEQCQESGASLVLLGKKKKKEEEGEGKKKGGGEGGAGADEEKEQSHRPFLMLQARQSEDHPHRRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCSHHHHHH

纯度

≥95% (SDS-PAGE)

蛋白标签

C-His

无载体

Yes

无动物源

Yes

偶联

Unconjugated

来源

重组表达

预测分子量

45 kDa

SDS-PAGE

13 - 16 kDa (mature) and 39 - 46 kDa (precursor) , under reducing conditions.

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4

复溶

Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。

图片

Protein Bioactivity: Recombinant Human Activin A Protein (rp142493)

Recombinant Human Activin A Protein (rp142493) - Protein Bioactivity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 2.87 ng/mL.

SDS-PAGE: Recombinant Human Activin A Protein (rp142493)

Recombinant Human Activin A Protein (rp142493) - SDS-PAGE
3 μg/lane of Recombinant Human Activin A Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 13 - 16 kDa (mature) and 39 - 46 kDa (precursor) under reducing condition. Under non-reducing conditions, the migration rate of the mature protein is 27 kDa, and the migration rate of the precursor protein is 39 - 46 kDa, and there may also be a migration rate of 55 - 65 kDa (indicating the combination of the precursor and mature forms). These changes in migration rate are mainly due to the effects of glycosylation and interchain disulfide bonds.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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批次质量数据。输入批号以获取对应 COA。

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产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1113619	分析证书	rp142493
ZJ24F1113620	分析证书	rp142493
ZJ24F1113621	分析证书	rp142493

技术文档和文章

Activin A、Noggin、DLL4与GM-CSF在iPSC定向分化研究中的应用

目录：技术文章

探索主题:

iPSC 诱导多能干细胞

Applications of Activin A, Noggin, DLL4, and GM-CSF in iPSC Directed Differentiation Research

目录：技术文章

探索主题:

GM-CSF myeloid differentiation iPSC Activin A Noggin DLL4 induced pluripotent stem cells directed differentiation germ layer induction BMP signaling Notch signaling functional maturation

含小胶质细胞脑类器官的构建、表征与神经疾病建模

目录：技术文章

探索主题:

阿尔茨海默病神经炎症小胶质细胞诱导多能干细胞三维培养脑类器官 MC-HBOs 帕金森病

Construction, Characterization, and Neurological Disease Modeling of Microglia-Containing Brain Organoids

目录：技术文章

探索主题:

Alzheimer’s Disease Parkinson’S Disease Modeling neuroinflammation Microglia induced pluripotent stem cells three-dimensional culture MC-HBOs brain organoids

激活素与抑制素的分子组成、信号调控及靶向治疗研究

目录：技术文章

探索主题:

靶向治疗纤维化 Activin A 激活素抑制素 INHBA ACVR1B ACVR2A ACVR2B 生殖调节

Molecular Composition, Signal Regulation, and Targeted Therapeutic Research of Activins and Inhibins

目录：技术文章

探索主题:

Targeted therapy fibrosis Activin A INHBA ACVR1B ACVR2A ACVR2B SMAD2/3 activins inhibins reproductive regulation

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计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

常见问题

本产品的纯度是多少？如何测定？

本产品的纯度为 ≥95%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。

什么是「无动物源」？

「无动物源」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

什么是「无载体」？

「无载体」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

本产品应如何储存？

请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。

本产品如何运输？

本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。

如何获取批次专用COA？

每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Bioactive 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 Azide Free 级别指南 → 查看 High Performance 级别指南 → 查看 His Tag 级别指南 →

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Recombinant Human Activin A Protein

储存与运输

质量文档

文献证明

概述

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

常见问题

需要帮助选择级别？

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