Recombinant Human EGF Protein

货号 (SKU)	包装规格	是否现货
rp145392-100μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	100μg	现货
rp145392-1mg	1mg	现货
rp145392-500μg	500μg	现货

基本描述

产品名称	Recombinant Human EGF Protein
别名	重组人表皮生长因子蛋白(EGF)
英文别名	beta-urogastrone \| EGF \| epidermal growth factor (beta-urogastrone) \| epidermal growth factor \| hEGF \| HOMG4 \| pro-epidermal growth factor \| URG \| Urogastrone \| EGF
规格或纯度	GMP, >95% SDS-PAGE, Active,Pichia pastoris, No tag, 971-1021aa
产品介绍	Purity ≥ 95% SDS-PAGE. ≥ 95 % HPLC. Additional sequence information N-terminal glycine. Full-length mature EGF chain. Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). Post-translational O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
生物活性	Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
内毒素水平	< 0.2 EU/µg
表达系统	Pichia pastoris
种属	Human
氨基酸	971-1021 aa
序列	NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWW ELR
纯度	＞95% SDS-PAGE
蛋白标签	No tag
N-末端序列	NSDSECPLSH
蛋白长度	Full length protein
无载体
Accession #	P01133
来源	Recombinant
预测分子量	6 kDa

产品规格参数

物理外观	Lyophilized
储存缓冲液	10mM磷酸钠缓冲液
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 10mM PBS to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	在-20℃下稳定至 24 个月，在 2~8℃下稳定至 4 周。配制为 100μg/ml 的储存液，2~8℃可稳定保存一周。若需延长储存时间，建议在-70℃或-20℃冷冻，不建议重复冷冻和解冻。

质检证书(COA)

质检报告(COA)

输入批号以搜索COA:

图片

Protein Bioactivity: Recombinant Human EGF Protein (rp145392)

Recombinant Human EGF Protein (rp145392)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.

SDS-PAGE: Recombinant Human EGF Protein (rp145392)

Recombinant Human EGF Protein (rp145392)-SDS-PAGE
5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 6.6 kDa.

产品问答

登录提交问题

查看全部

您提交该产品问题后,我们会在1-2个工作日内给您答复,您可以登录"我的账号",然后点击"我的产品问答"查看答案

参考文献

1. Ogiso H, Ishitani R, Nureki O, Fukai S, Yamanaka M, Kim JH, Saito K, Sakamoto A, Inoue M, Shirouzu M et al.. (2002) Crystal structure of the complex of human epidermal growth factor and receptor extracellular domains.. Cell, 110 (6): (775-87). [PMID:12297050]
2. Feng Y, Yin Y, Weiser A, Griffin E, Cameron MD, Lin L, Ruiz C, Schürer SC, Inoue T, Rao PV, Schröter T, Lograsso P.. (2008) Discovery of substituted 4-(pyrazol-4-yl)-phenylbenzodioxane-2-carboxamides as potent and highly selective Rho kinase (ROCK-II) inhibitors.. J Med Chem, 51 (21): (6642-6645). [PMID:18834107]
3. Lesuisse D, Mauger J, Nemecek C, Maignan S, Boiziau J, Harlow G, Hittinger A, Ruf S, Strobel H, Nair A, Ritter K, Malleron JL, Dagallier A, El-Ahmad Y, Guilloteau JP, Guizani H, Bouchard H, Venot C.. (2011) Discovery of the first non-ATP competitive IGF-1R kinase inhibitors: advantages in comparison with competitive inhibitors.. Bioorg Med Chem Lett, 21 (8): (2224-2228). [PMID:21441024]
4. Hodgetts KJ, Ge P, Yoon T, De Lombaert S, Brodbeck R, Gulianello M, Kieltyka A, Horvath RF, Kehne JH, Krause JE, Maynard GD, Hoffman D, Lee Y, Fung L, Doller D.. (2011) Discovery of N-(1-ethylpropyl)-[3-methoxy-5-(2-methoxy-4-trifluoromethoxyphenyl)-6-methyl-pyrazin-2-yl]amine 59 (NGD 98-2): an orally active corticotropin releasing factor-1 (CRF-1) receptor antagonist.. J Med Chem, 54 (12): (4187-4206). [PMID:21618986]
5. Gregory, H H and Preston, B M BM.. (1977) The primary structure of human urogastrone.. International journal of peptide and protein research, (): (). [PMID:300079]
6. Hommel, U U, Harvey, T S TS, Driscoll, P C PC and Campbell, I D ID.. (1992) Human epidermal growth factor. High resolution solution structure and comparison with human transforming growth factor alpha.. Journal of molecular biology, (5): (). [PMID:1522591]
7. Furuya, M M, Akashi, S S and Hirayama, K K.. (1989) The primary structure of human EGF produced by genetic engineering, studied by high-performance tandem mass spectrometry.. Biochemical and biophysical research communications, (15): (). [PMID:2789514]
8. Bell, G I GI and 8 more authors.. (1986) Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization.. Nucleic acids research, (11): (). [PMID:3491360]
9. Lu, H S HS and 5 more authors.. (2001) Crystal structure of human epidermal growth factor and its dimerization.. The Journal of biological chemistry, (14): (). [PMID:11438527]
10. Ferguson, Kathryn M KM and 5 more authors.. (2003) EGF activates its receptor by removing interactions that autoinhibit ectodomain dimerization.. Molecular cell, (): (). [PMID:12620237]
11. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): (). [PMID:15815621]
12. Groenestege, Wouter M Tiel WM and 10 more authors.. (2007) Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia.. The Journal of clinical investigation, (): (). [PMID:17671655]
13. Lu, Chafen C and 6 more authors.. (2010) Structural evidence for loose linkage between ligand binding and kinase activation in the epidermal growth factor receptor.. Molecular and cellular biology, (): (). [PMID:20837704]
14. Huang, Hsiao-Wen HW, Mohan, Sepuru K SK and Yu, C C.. (2010) The NMR solution structure of human epidermal growth factor (hEGF) at physiological pH and its interactions with suramin.. Biochemical and biophysical research communications, (26): (). [PMID:21029725]
15. Zettl, Markus M, Adrain, Colin C, Strisovsky, Kvido K, Lastun, Viorica V and Freeman, Matthew M.. (2011) Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling.. Cell, (1): (). [PMID:21439629]

Recombinant Human EGF Protein

库存信息

库存信息

库存信息

基本描述

产品规格参数

质检证书(COA)

质检报告(COA)

图片

相关文档

质检报告COA

相关技术文章

产品问答

产品问答

参考文献