Recombinant Human EGF Protein

2篇引用文献

无动物源

≥98%(SDS-PAGE)

功能和特点

表达系统: 毕赤酵母
Accession #: P01133
蛋白标签: No tag
生物活性: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
内毒素水平: <1.0 EU/mg

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货号 (SKU)	包装规格	是否现货
rp155969-50μg	50μg	现货
rp155969-200μg	200μg	现货
rp155969-1mg	1mg	现货

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点击查看蛋白信息: EGF

Accession#:P01133 (9) EGF (9) Gene ID:1950 (9)

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基本描述

产品名称	Recombinant Human EGF Protein
别名	重组人EGF蛋白
英文别名	UNII-TZK30RF92W \| Human EGF \| MG 111 \| CCRIS 6735 \| Nepidermin [INN] \| NEPIDERMIN \| Human epidermal growth factor \| TZK30RF92W \| beta-Urogastrone (human) \| Epidermal growth factor (human) \| AKOS040759488 \| DTXSID80211314 \| EGF
规格或纯度	Animal Free, Carrier Free, GMP, Low Endotoxin, High performance, 生物活性级, ≥98%(SDS-PAGE)
生化机理	EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
产品介绍	pH值：6.5-7.5 人表皮生长因子（EGF）分子量为6.5kD，含有53个氨基酸，分子内有3个二硫键。EGF与位于细胞表面的特异受体EGFR 结合后，引起细胞内一系列的生化反应：提高细胞内的钙离子浓度水平，促进糖酵解的进行，增加蛋白质的合成，并且可以增强EGFR这一类特定基因的表达来促进DNA 的合成和细胞的增殖。
生物活性	Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
内毒素水平	<1.0 EU/mg
表达系统	毕赤酵母
种属	人(Human)
氨基酸	971-1023 aa
序列	NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
纯度	≥95% (SDS-PAGE)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
Accession #	P01133
来源	重组表达
预测分子量	6.5kD
SDS-PAGE	7.1 kDa, under reducing conditions.

AI解读

图片

Protein Bioactivity: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1ng/mL.

SDS-PAGE: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-SDS-PAGE
5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 7.1 kDa.

产品规格参数

物理形态	冻干(Lyophilized)
复溶	Reconstitute in sterile water to a concentration of 100-200μg/ml, and further dilute it in other solvents. Upon reconstitution, the product could be stored at 4 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20℃ 下保存 3 年。收货后建议分装。避免冷冻/解冻循环。
CAS编号和信息	62253-63-8
分子类型	蛋白质

安全和危险性（GHS）

象形图	GHS07
信号词	Warning
危险声明	H315: 引起皮肤刺激 H319: 引起严重眼睛刺激 H335: 可能引起呼吸道刺激
预防措施声明	P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P302+P352: 如皮肤沾染：用水充分清洗。 P321: 特殊处理（请参阅此标签上的...）。 P405: 密闭存放 P501: 将内容物/容器处理到。。。 P264: 处理后要彻底洗手。 P271: 仅在室外或通风良好的地方使用。 P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。 P403+P233: 存放在通风良好的地方。保持容器密闭。 P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。 P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。 P337+P317: 如果眼睛刺激持续：寻求医疗帮助。 P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。 P319: 如果你感到不适，请寻求医疗帮助。
WGK Germany	3

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找到10个结果

批号(Lot Number)	证书类型	日期	货号
ZJ24F1215895	分析证书	24-12-26	rp155969
ZJ24F1215957	分析证书	24-12-26	rp155969
ZJ24F0607416	分析证书	24-06-28	rp155969
ZJ24F0505983	分析证书	24-05-23	rp155969
ZJ24F0505244	分析证书	24-05-09	rp155969
ZJ24F0303826	分析证书	24-03-29	rp155969
ZJ24F0303825	分析证书	24-03-29	rp155969
ZJ23F0300039	分析证书	23-11-10	rp155969
ZJ24F0505243	分析证书	23-11-10	rp155969
ZJ23F0300040	分析证书	23-03-13	rp155969

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参考文献

1. Ogiso H, Ishitani R, Nureki O, Fukai S, Yamanaka M, Kim JH, Saito K, Sakamoto A, Inoue M, Shirouzu M et al.. (2002) Crystal structure of the complex of human epidermal growth factor and receptor extracellular domains.. Cell, 110 (6): (775-87). [PMID:12297050] [10.1021/op500134e]
2. Feng Y, Yin Y, Weiser A, Griffin E, Cameron MD, Lin L, Ruiz C, Schürer SC, Inoue T, Rao PV, Schröter T, Lograsso P.. (2008) Discovery of substituted 4-(pyrazol-4-yl)-phenylbenzodioxane-2-carboxamides as potent and highly selective Rho kinase (ROCK-II) inhibitors.. J Med Chem, 51 (21): (6642-6645). [PMID:18834107] [10.1021/jm800986w]
3. Lesuisse D, Mauger J, Nemecek C, Maignan S, Boiziau J, Harlow G, Hittinger A, Ruf S, Strobel H, Nair A, Ritter K, Malleron JL, Dagallier A, El-Ahmad Y, Guilloteau JP, Guizani H, Bouchard H, Venot C.. (2011) Discovery of the first non-ATP competitive IGF-1R kinase inhibitors: advantages in comparison with competitive inhibitors.. Bioorg Med Chem Lett, 21 (8): (2224-2228). [PMID:21441024] [10.1016/j.bmcl.2011.03.003]
4. Gregory, H H and Preston, B M BM.. (1977) The primary structure of human urogastrone.. International journal of peptide and protein research, [PMID:300079]
5. Hommel, U U, Harvey, T S TS, Driscoll, P C PC and Campbell, I D ID.. (1992) Human epidermal growth factor. High resolution solution structure and comparison with human transforming growth factor alpha.. Journal of molecular biology, (5): [PMID:1522591]
6. Furuya, M M, Akashi, S S and Hirayama, K K.. (1989) The primary structure of human EGF produced by genetic engineering, studied by high-performance tandem mass spectrometry.. Biochemical and biophysical research communications, (15): [PMID:2789514]
7. Bell, G I GI and 8 more authors.. (1986) Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization.. Nucleic acids research, (11): [PMID:3491360]
8. Lu, H S HS and 5 more authors.. (2001) Crystal structure of human epidermal growth factor and its dimerization.. The Journal of biological chemistry, (14): [PMID:11438527]
9. Ferguson, Kathryn M KM and 5 more authors.. (2003) EGF activates its receptor by removing interactions that autoinhibit ectodomain dimerization.. Molecular cell, [PMID:12620237]
10. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621]
11. Groenestege, Wouter M Tiel WM and 10 more authors.. (2007) Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia.. The Journal of clinical investigation, [PMID:17671655]
12. Lu, Chafen C and 6 more authors.. (2010) Structural evidence for loose linkage between ligand binding and kinase activation in the epidermal growth factor receptor.. Molecular and cellular biology, [PMID:20837704]
13. Huang, Hsiao-Wen HW, Mohan, Sepuru K SK and Yu, C C.. (2010) The NMR solution structure of human epidermal growth factor (hEGF) at physiological pH and its interactions with suramin.. Biochemical and biophysical research communications, (26): [PMID:21029725]
14. Zettl, Markus M, Adrain, Colin C, Strisovsky, Kvido K, Lastun, Viorica V and Freeman, Matthew M.. (2011) Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling.. Cell, (1): [PMID:21439629]
15. Cao Mingbo, Ren Yupeng, Li Yuxuan, Deng Junfeng, Su Xiaorui, Tang Yongchang, Yuan Feng, Deng Haixia, Yang Gaoyuan, He Zhiwei, Liu Bo, Yao Zhicheng, Deng Meihai. (2023) Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis. International Journal of Biological Sciences, 19 (12): (3678-3693). [PMID:37564197] [10.7150/ijbs.85270]
16. Maoze Guo, Yuqiu Wang, Bingbing Gao, Bingfang He. (2021) Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management. ACS Nano, 15 (9): (15316–15327). [PMID:34533924] [10.1021/acsnano.1c06279]

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Recombinant Human EGF Protein

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基本描述

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产品规格参数

安全和危险性（GHS）

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