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Recombinant Human GM-CSF Protein (rp156011) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is typically 0.82 ng/mL.
His标签,PBS Only,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description:
GM CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy induced neutropenia, and GM CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM CSF, similar to IL 3 and IL 5, is a cytokine with a core of four bundled alpha -helices. Mature human GM CSF shares 63% 70% amino acid sequence identity with canine, feline, porcine, and rat GM CSF and 54% with mouse GM CSF. GM CSF exerts its biological effects through a heterodimeric receptor complex composed of GM CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high affinity receptors for IL 3 and IL 5. In addition, GM CSF binds a naturally occurring soluble form of GM CSF R alpha. Human GM CSF is active on canine and feline cells but not on murine cells.
Recombinant Human GM-CSF Protein (rp156011) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is typically 0.82 ng/mL.
Recombinant Human GM-CSF Protein (rp156011) - SDS-PAGE
3 μg/lane of Recombinant Human GM-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.8 kDa.
Recombinant Human GM-CSF Protein (rp156011) - ELISA
Immobilized Recombinant Human GM-CSF Protein (rp156011) at 1.0 μg/mL can bind Namilumab (anti-GM-CSF) (Ab175608) with the EC50 of 62.67 ng/mL.
Recombinant Human GM-CSF Protein (rp156011) - ELISA
Immobilized Recombinant Human GM-CSF Protein (rp156011) at 1.0 μg/mL can bind Gimsilumab (anti-GM-CSF) (Ab175548) with the EC50 of 23.52 ng/mL.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp156011 | |
| 分析证书 | rp156011 | |
| 分析证书 | rp156011 |
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