Recombinant Human GM-CSF Protein

货号: rp156011
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥95%(SDS-PAGE)
Accession #
P04141
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 0.82 ng/mL.
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规格
库存
价格
数量
10μg
rp156011-10μg
现货 Stock Image
¥699.90
50μg
rp156011-50μg
现货 Stock Image
¥2,199.90
100μg
rp156011-100μg
现货 Stock Image
¥3,499.90
1mg
rp156011-1mg
期货 Stock Image
¥15,999.90
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为什么选择此级别

His标签,PBS Only,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description:
GM CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy induced neutropenia, and GM CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM CSF, similar to IL 3 and IL 5, is a cytokine with a core of four bundled alpha -helices. Mature human GM CSF shares 63% 70% amino acid sequence identity with canine, feline, porcine, and rat GM CSF and 54% with mouse GM CSF. GM CSF exerts its biological effects through a heterodimeric receptor complex composed of GM CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high affinity receptors for IL 3 and IL 5. In addition, GM CSF binds a naturally occurring soluble form of GM CSF R alpha. Human GM CSF is active on canine and feline cells but not on murine cells.

规格

产品名称
Recombinant Human GM-CSF Protein
别名
重组人GM-CSF蛋白 | 重组人粒细胞巨噬细胞集落刺激因子 | 重组人 GM-CSF 蛋白
英文别名
Molgramostim | molgramostin | Sargramostim | colony stimulating factor 2 (granulocyte-macrophage) | Colony-stimulating factor | CSF | CSF2 | CSF-2 | GMCSF | GM-CSF | GMCSFgranulocyte-macrophage colony-stimulating factor | granulocyte-macrophage colony sti
规格或纯度
无载体,生物活性,高性能,His标签,PBS Only,≥95%(SDS-PAGE)
生物活性
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 0.82 ng/mL.
内毒素水平
<1.0 EU/μg
表达系统
E. coli
种属
人(Human)
氨基酸
18-144 aa
序列
MGSSHHHHHHSSGLVPRGSHMAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
蛋白标签
N-His
无载体
Yes
无动物源
No
Accession #
来源
重组表达
预测分子量
16.8 kDa
SDS-PAGE
14.8 kDa, under reducing conditions; 14.8 kDa, under non-reducing condition
分子类型
蛋白质
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片

Recombinant Human GM-CSF Protein (rp156011) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is typically 0.82 ng/mL.

Recombinant Human GM-CSF Protein (rp156011) - SDS-PAGE
3 μg/lane of Recombinant Human GM-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.8 kDa.

Recombinant Human GM-CSF Protein (rp156011) - ELISA
Immobilized Recombinant Human GM-CSF Protein (rp156011) at 1.0 μg/mL can bind Namilumab (anti-GM-CSF) (Ab175608) with the EC50 of 62.67 ng/mL.

Recombinant Human GM-CSF Protein (rp156011) - ELISA
Immobilized Recombinant Human GM-CSF Protein (rp156011) at 1.0 μg/mL can bind Gimsilumab (anti-GM-CSF) (Ab175548) with the EC50 of 23.52 ng/mL.

技术文档

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高级数据

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0708343 分析证书 rp156011
ZJ24F0708344 分析证书 rp156011
ZJ24F0708345 分析证书 rp156011
技术文档和文章
溶液计算器