Recombinant Human IL-10 Protein

货号: rp156176
有货
级别和纯度: 无动物源 ? 无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥90%(SDS-PAGE) 见COA
Accession #
P22301
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL. Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.
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规格
库存
价格
数量
10μg
rp156176-10μg
现货 Stock Image
¥569.90
50μg
rp156176-50μg
现货 Stock Image
¥1,999.90
100μg
rp156176-100μg
现货 Stock Image
¥3,299.90
1mg
rp156176-1mg
期货 Stock Image
¥15,999.90
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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无动物源,无载体,生物活性 级 提供 ≥90%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Human IL-10 Protein
别名
重组人IL-10蛋白 | 重组人白细胞介素-10蛋白
英文别名
CSIF | CSIFMGC126450 | Cytokine synthesis inhibitory factor | GVHDS | IL10 | IL-10 | IL10A | IL-10MGC126451 | interleukin 10 | interleukin-10 | TGIF
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,His标签,PBS Only,≥90%(SDS-PAGE),见COA
生化机理
Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155. IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature human IL‑10 shares 72% ‑ 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells. IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction. IL‑10 R beta also associates with IL‑20 R alpha, IL‑22 R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29. IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
生物活性
Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL. Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.
表达系统
HEK293
种属
人(Human)
氨基酸
19-178 aa
序列
SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRNHHHHHH​
蛋白标签
C-His
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
19.5 kDa
SDS-PAGE
17.4 kDa, under reducing conditions; 17.7 & 16.3 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片

Recombinant Human IL-10 Protein (rp156176) - Binding Activity
Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL.


Recombinant Human IL-10 Protein (rp156176) - ELISA
Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.

Recombinant Human IL-10 Protein (rp156176) - SDS-PAGE
3 μg/lane of Recombinant Human IL-10 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.4 kDa under reducing conditions and 17.7 & 16.3 kDa under non-reducing conditions.

技术文档

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全面的危险、操作、储存及法规合规文件。

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高级数据

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ25F0825296 分析证书 rp156176
ZJ25F0825297 分析证书 rp156176
ZJ25F0825298 分析证书 rp156176
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白介素受体信号的JAK-STAT及NF-κB轴机制解析与检测模型及转化边界梳理
Interleukin Receptor Signaling: Mechanistic Analysis of the JAK-STAT and NF-κB Axes with an Overview of Assays, Experimental Models, and Translational Boundaries
TH细胞分化通路的激活基础、谱系决定与功能分化
Activation Basis, Lineage Commitment, and Functional Differentiation of TH Cell Differentiation Pathways
单宁酸-透钙磷石原位复合骨水泥的制备策略与体外生物性能评价
Preparation Strategy and In Vitro Biological Performance Evaluation of Tannic Acid–Brushite In Situ Composite Bone Cement
溶液计算器