Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL. Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.
Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155. IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature human IL‑10 shares 72% ‑ 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells. IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction. IL‑10 R beta also associates with IL‑20 R alpha, IL‑22 R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29. IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
生物活性
Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL. Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.
17.4 kDa, under reducing conditions; 17.7 & 16.3 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human IL-10 Protein (rp156176) - Binding Activity Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL.
Recombinant Human IL-10 Protein (rp156176) - ELISA Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.
Recombinant Human IL-10 Protein (rp156176) - SDS-PAGE 3 μg/lane of Recombinant Human IL-10 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.4 kDa under reducing conditions and 17.7 & 16.3 kDa under non-reducing conditions.