Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Post-translational: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
生物活性
Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is typically 8.2 ng/mL.
18.0-25.0 kDa, under reducing conditions; 38.9-53.3 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 4mM HCl.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human VEGFA-165 Protein (rp156339) - Protein Bioactivity Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is typically 8.2 ng/mL.
Recombinant Human VEGFA-165 Protein (rp156339) - SDS-PAGE 3 μg/lane of Recombinant Human VEGFA-165 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 18.0-25.0 kDa under reducing conditions and 38.9-53.3 kDa under non-reducing conditions. There are multiple pairs of disulfide bonds in the protein sequence. Disulfide bonds cause the protein to present as a polymer in a non-reducing conditions (Uniprot ID:P15692-4).